Copper homeostasis gene discovery in Drosophila melanogaster

Melanie Norgate, Adam Southon, Sige Zou, Ming Zhan, Yu Sun, Phil Batterham, James Camakaris

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Recent studies have shown a high level of conservation between Drosophila melanogaster and mammalian copper homeostasis mechanisms. These studies have also demonstrated the efficiency with which this species can be used to characterize novel genes, at both the cellular and whole organism level. As a versatile and inexpensive model organism, Drosophila is also particularly useful for gene discovery applications and thus has the potential to be extremely useful in identifying novel copper homeostasis genes and putative disease genes. In order to assess the suitability of Drosophila for this purpose, three screening approaches have been investigated. These include an analysis of the global transcriptional response to copper in both adult flies and an embryonic cell line using DNA microarray analysis. Two mutagenesis-based screens were also utilized. Several candidate copper homeostasis genes have been identified through this work. In addition, the results of each screen were carefully analyzed to identify any factors influencing efficiency and sensitivity. These are discussed here with the aim of maximizing the efficiency of future screens and the most suitable approaches are outlined. Building on this information, there is great potential for the further use of Drosophila for copper homeostasis gene discovery.

Original languageEnglish (US)
Pages (from-to)683-697
Number of pages15
JournalBioMetals
Volume20
Issue number3-4
DOIs
StatePublished - Jun 1 2007

Keywords

  • Copper
  • Drosophila melanogaster
  • Microarray
  • Mutagenesis
  • Screen

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Copper homeostasis gene discovery in Drosophila melanogaster'. Together they form a unique fingerprint.

Cite this