TY - JOUR
T1 - Continuous aqueous two-phase extraction of microalgal C-phycocyanin using a coiled flow inverter
AU - Ruiz-Ruiz, Federico
AU - López-Guajardo, Enrique
AU - Vázquez-Villegas, Patricia
AU - del Angel-Chong, Martha E.
AU - Nigam, K. D.P.
AU - Willson, Richard C.
AU - Rito-Palomares, Marco
N1 - Funding Information:
The authors wish to acknowledge the financial support of Tecnologico de Monterrey through the grants given to the Bioprocess and Synthetic Biology Research Group ( 002EICIP01 ) and to the Energy and Climate Change Research Group from the School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey, Mexico. Enrique Lopez-Guajardo and K.D.P. Nigam wish to acknowledge Project 266632 “Laboratorio Binacional para la Gestión Inteligente de la Sustentabilidad Energética y la Formación Tecnológica” [“Bi-National Laboratory on Smart Sustainable Energy Management and Technology Training”], funded by the CONACYT SENER Fund for Energy Sustainability (Agreement: S0019-2014-01 ). The authors would like to acknowledge Mr. Juan Cristerna Torres and Master Juan Cristerna Villegas for the design and 3D printing (additive manufacturing) of the settler (based on the single-stage mixer-settler unit design presented in [ 14 ] and the frame for the CFI used in this research and Ms. Daniela Montealegre-Mariscal for her support on the preparation of the laboratory materials.
Publisher Copyright:
© 2019
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/8
Y1 - 2019/8
N2 - In this study, the intensification of C-phycocyanin protein extraction from the green microalgae Spirulina maxima was successfully achieved using aqueous two-phase systems coupled to different continuous-operation extractors. Three systems with different total concentrations of Polyethylene glycol and potassium phosphate salt were tested in three different types of continuous systems: a conventional straight-tube extractor, a helical-coiled extractor, and a coiled flow inverter extractor. Results showed that the coiled flow inverter extractor achieves higher overall performance than the other continuous extractors at the same flow rate and same residence time for all the concentrations tested. Similarly, all the continuous-operation extractors showed higher extraction performance than a batch-operation extractor for similar times tested. It was observed that extraction performance in the coiled flow inverter increases as flow rate increases (coefficient of partition increases 1.74 fold by increasing the flow rate from 10 mL/min to 30 mL/min). The C-phycocyanin extraction in this continuous system was benefited from a decrease in the extraction time with high Dean numbers.
AB - In this study, the intensification of C-phycocyanin protein extraction from the green microalgae Spirulina maxima was successfully achieved using aqueous two-phase systems coupled to different continuous-operation extractors. Three systems with different total concentrations of Polyethylene glycol and potassium phosphate salt were tested in three different types of continuous systems: a conventional straight-tube extractor, a helical-coiled extractor, and a coiled flow inverter extractor. Results showed that the coiled flow inverter extractor achieves higher overall performance than the other continuous extractors at the same flow rate and same residence time for all the concentrations tested. Similarly, all the continuous-operation extractors showed higher extraction performance than a batch-operation extractor for similar times tested. It was observed that extraction performance in the coiled flow inverter increases as flow rate increases (coefficient of partition increases 1.74 fold by increasing the flow rate from 10 mL/min to 30 mL/min). The C-phycocyanin extraction in this continuous system was benefited from a decrease in the extraction time with high Dean numbers.
KW - Aqueous two-phase system
KW - C-phycocyanin
KW - Coiled flow inverter
KW - Continuous extraction
KW - Microalgae
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U2 - 10.1016/j.cep.2019.107554
DO - 10.1016/j.cep.2019.107554
M3 - Article
AN - SCOPUS:85066609038
VL - 142
JO - Chemical Engineering and Processing: Process Intensification
JF - Chemical Engineering and Processing: Process Intensification
SN - 0255-2701
M1 - 107554
ER -