TY - JOUR
T1 - Construction of amphiphilic copolymer nanoparticles based on hyperbranched Poly (Amine-Ester) and 1,2-Dipalmitoyl-Sn-Glycero-3-Phosphoethanolamine as drug carriers for cancer therapy
AU - Wu, Yan
AU - Jiao, Fang
AU - Han, Siyuan
AU - Fan, Tengfei
AU - Liu, Ying
AU - Li, Wei
AU - Hu, Liming
AU - Zhao, Yuliang
AU - Chen, Chunying
N1 - Funding Information:
The authors acknowledge the financial support from the Ministry of Science and Technology of China ( 2010CB934004 and 2011CB933401 ), National Natural Science Foundation of China ( 31070854 ), the CAS Knowledge Innovation Program and Postdoctoral Foundation of China ( 20100480465 ).
PY - 2011/12
Y1 - 2011/12
N2 - Novel amphiphilic copolymer nanoparticles (HPAE-co-PLA-DPPE) composed of hyperbranched poly (amine-ester), polylactide and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) segments were designed and synthesized that provided high encapsulation efficiency. These nanoparticles (NPs) were used to encapsulate an antitumor model drug, doxorubicin (DOX). The resulting NPs exhibited high encapsulation efficiency to DOX under an appropriate condition. In vitro release experiments revealed that the release of DOX from NPs was faster at pH 4.5 than that at pH 7.4 or pH 6.0. Confocal microscopy observation indicated that the DOX-loaded NPs can enter cells and localize in lysosomes that can be released quickly into the cytoplasm. The DOX-loaded NPs showed comparable anticancer efficacy with the free drug both in vivo and in vitro. These results demonstrate a feasible application of the hyperbranched copolymer, HPAE-co-PLA-DPPE, as a promising nanocarrier for intracellular delivery of antitumor drugs. From the Clinical Editor: In this paper, the development of novel amphiphilic copolymer nanoparticles is discussed with the goal of establishing high encapsulation efficiency for chemotherapy drugs.
AB - Novel amphiphilic copolymer nanoparticles (HPAE-co-PLA-DPPE) composed of hyperbranched poly (amine-ester), polylactide and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) segments were designed and synthesized that provided high encapsulation efficiency. These nanoparticles (NPs) were used to encapsulate an antitumor model drug, doxorubicin (DOX). The resulting NPs exhibited high encapsulation efficiency to DOX under an appropriate condition. In vitro release experiments revealed that the release of DOX from NPs was faster at pH 4.5 than that at pH 7.4 or pH 6.0. Confocal microscopy observation indicated that the DOX-loaded NPs can enter cells and localize in lysosomes that can be released quickly into the cytoplasm. The DOX-loaded NPs showed comparable anticancer efficacy with the free drug both in vivo and in vitro. These results demonstrate a feasible application of the hyperbranched copolymer, HPAE-co-PLA-DPPE, as a promising nanocarrier for intracellular delivery of antitumor drugs. From the Clinical Editor: In this paper, the development of novel amphiphilic copolymer nanoparticles is discussed with the goal of establishing high encapsulation efficiency for chemotherapy drugs.
KW - Anti-tumor activity
KW - Biocompatibility
KW - Controlled drug release
KW - Drug delivery
KW - Hyperbranched copolymer
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U2 - 10.1016/j.nano.2011.04.010
DO - 10.1016/j.nano.2011.04.010
M3 - Article
C2 - 21664985
AN - SCOPUS:82255191847
VL - 7
SP - 945
EP - 954
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
SN - 1549-9634
IS - 6
ER -