Constraint factor graph cut-based active contour method for automated cellular image segmentation in RNAi screening

C. Chen, H. Li, X. Zhou, S. T C Wong

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Image-based, high throughput genome-wide RNA interference (RNAi) experiments are increasingly carried out to facilitate the understanding of gene functions in intricate biological processes. Automated screening of such experiments generates a large number of images with great variations in image quality, which makes manual analysis unreasonably time-consuming. Therefore, effective techniques for automatic image analysis are urgently needed, in which segmentation is one of the most important steps. This paper proposes a fully automatic method for cells segmentation in genome-wide RNAi screening images. The method consists of two steps: nuclei and cytoplasm segmentation. Nuclei are extracted and labelled to initialize cytoplasm segmentation. Since the quality of RNAi image is rather poor, a novel scale-adaptive steerable filter is designed to enhance the image in order to extract long and thin protrusions on the spiky cells. Then, constraint factor GCBAC method and morphological algorithms are combined to be an integrated method to segment tight clustered cells. Compared with the results obtained by using seeded watershed and the ground truth, that is, manual labelling results by experts in RNAi screening data, our method achieves higher accuracy. Compared with active contour methods, our method consumes much less time. The positive results indicate that the proposed method can be applied in automatic image analysis of multi-channel image screening data.

Original languageEnglish (US)
Pages (from-to)177-191
Number of pages15
JournalJournal of Microscopy
Volume230
Issue number2
DOIs
StatePublished - May 2008

Keywords

  • Active contour
  • Automatic image segmentation
  • Constraint factor
  • Fluorescent microscopy
  • Genome-wide screening
  • Graph cut
  • Morphological algorithm
  • RNAi

ASJC Scopus subject areas

  • Instrumentation

Fingerprint Dive into the research topics of 'Constraint factor graph cut-based active contour method for automated cellular image segmentation in RNAi screening'. Together they form a unique fingerprint.

Cite this