Conserved sequence analysis of influenza a virus ha segment and its application in rapid typing

Qianyu Lin; Xiang Ji; Feng Wu; Lan Ma

doi:10.3390/diagnostics11081328

Conserved sequence analysis of influenza a virus ha segment and its application in rapid typing

Qianyu Lin, Xiang Ji, Feng Wu, Lan Ma

Houston Methodist

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The high mutation rate of the influenza A virus hemagglutinin segment poses great challenges to its long-term effective testing and subtyping. Our conserved sequence searching method achieves high-specificity conserved sequences on H1–H9 subtypes. In addition, PCR experiments show that primers based on conserved sequences can be used in influenza A virus HA subtyping. Conserved sequence-based primers are expected to be long-term, effective subtyping tools for influenza A virus HA.

Original language	English (US)
Article number	1328
Journal	Diagnostics
Volume	11
Issue number	8
DOIs	https://doi.org/10.3390/diagnostics11081328
State	Published - Jul 23 2021

Keywords

Conserved sequence
Influenza
Multiple sequence alignment
Rapid detection

ASJC Scopus subject areas

Clinical Biochemistry

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10.3390/diagnostics11081328

Cite this

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title = "Conserved sequence analysis of influenza a virus ha segment and its application in rapid typing",

abstract = "The high mutation rate of the influenza A virus hemagglutinin segment poses great challenges to its long-term effective testing and subtyping. Our conserved sequence searching method achieves high-specificity conserved sequences on H1–H9 subtypes. In addition, PCR experiments show that primers based on conserved sequences can be used in influenza A virus HA subtyping. Conserved sequence-based primers are expected to be long-term, effective subtyping tools for influenza A virus HA.",

keywords = "Conserved sequence, Influenza, Multiple sequence alignment, Rapid detection",

author = "Qianyu Lin and Xiang Ji and Feng Wu and Lan Ma",

note = "Funding Information: Funding: This work was supported by the National Key R \& D Plan in China (2016YFD0501103), Shenzhen strategic emerging industry development special funds (JCYJ20170816143646446), Shen-zhen Science and Technology research and development funds (JCYJ20200109143018683). Funding Information: This work was supported by the National Key R \& D Plan in China (2016YFD0501103), Shenzhen strategic emerging industry development special funds (JCYJ20170816143646446), Shenzhen Science and Technology research and development funds (JCYJ20200109143018683). Publisher Copyright: {\textcopyright} 2021 by the authors.",

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doi = "10.3390/diagnostics11081328",

language = "English (US)",

volume = "11",

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T1 - Conserved sequence analysis of influenza a virus ha segment and its application in rapid typing

AU - Lin, Qianyu

AU - Ji, Xiang

AU - Wu, Feng

AU - Ma, Lan

N1 - Funding Information: Funding: This work was supported by the National Key R & D Plan in China (2016YFD0501103), Shenzhen strategic emerging industry development special funds (JCYJ20170816143646446), Shen-zhen Science and Technology research and development funds (JCYJ20200109143018683). Funding Information: This work was supported by the National Key R & D Plan in China (2016YFD0501103), Shenzhen strategic emerging industry development special funds (JCYJ20170816143646446), Shenzhen Science and Technology research and development funds (JCYJ20200109143018683). Publisher Copyright: © 2021 by the authors.

PY - 2021/7/23

Y1 - 2021/7/23

N2 - The high mutation rate of the influenza A virus hemagglutinin segment poses great challenges to its long-term effective testing and subtyping. Our conserved sequence searching method achieves high-specificity conserved sequences on H1–H9 subtypes. In addition, PCR experiments show that primers based on conserved sequences can be used in influenza A virus HA subtyping. Conserved sequence-based primers are expected to be long-term, effective subtyping tools for influenza A virus HA.

AB - The high mutation rate of the influenza A virus hemagglutinin segment poses great challenges to its long-term effective testing and subtyping. Our conserved sequence searching method achieves high-specificity conserved sequences on H1–H9 subtypes. In addition, PCR experiments show that primers based on conserved sequences can be used in influenza A virus HA subtyping. Conserved sequence-based primers are expected to be long-term, effective subtyping tools for influenza A virus HA.

KW - Conserved sequence

KW - Influenza

KW - Multiple sequence alignment

KW - Rapid detection

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JO - Diagnostics

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ER -

Conserved sequence analysis of influenza a virus ha segment and its application in rapid typing

Abstract

Keywords

ASJC Scopus subject areas

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