Conditional control of selectin ligand expression and global fucosylation events in mice with a targeted mutation at the FX locus

Peter L. Smith, Jay T. Myers, Clare E. Rogers, Lan Zhou, Bronia Petryniak, Daniel J. Becker, Jonathon W. Homeister, John B. Lowe

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

Glycoprotein fucosylation enables fringe-dependent modulation of signal transduction by Notch transmembrane receptors, contributes to selectindependent leukocyte trafficking, and is faulty in leukocyte adhesion deficiency (LAD) type II, also known as congenital disorder of glycosylation (CDG)-IIc, a rare human disorder characterized by psychomotor defects, developmental abnormalities, and leukocyte adhesion defects. We report here that mice with an induced null mutation in the FX locus, which encodes an enzyme in the de novo pathway for GDP-fucose synthesis, exhibit a virtually complete deficiency of cellular fucosylation, and variable frequency of intrauterine demise determined by parental FX genotype. Live-born FX(-/-) mice exhibit postnatal failure to thrive that is suppressed with a fucose-supplemented diet. FX(-/-) adults suffer from an extreme neutrophilia, myeloproliferation, and absence of leukocyte selectin ligand expression reminiscent of LAD-II/CDG-IIc. Contingent restoration of leukocyte and endothelial selectin ligand expression, general cellular fucosylation, and normal postnatal physiology is achieved by modulating dietary fucose to supply a salvage pathway for GDP-fucose synthesis. Conditional control of fucosylation in FX(-/-) mice identifies cellular fucosylation events as essential concomitants to fertility, early growth and development, and leukocyte adhesion.

Original languageEnglish (US)
Pages (from-to)801-815
Number of pages15
JournalJournal of Cell Biology
Volume158
Issue number4
DOIs
StatePublished - Aug 19 2002

Keywords

  • CDG-IIc
  • Fucosylation
  • GDP-fucose
  • LAD-II
  • Selectin

ASJC Scopus subject areas

  • Cell Biology

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