Concurrent generation of functional smooth muscle and endothelial cells via a vascular progenitor

Melanie Marchand, Erica K. Anderson, Smruti M. Phadnis, Michael T. Longaker, John P. Cooke, Bertha Chen, Renee A. Reijo Pera

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Smooth muscle cells (SMCs) and endothelial cells (ECs) are typically derived separately, with low efficiencies, fromhumanpluripotent stemcells (hPSCs).The concurrent generation of these cell typesmight leadtopotentialapplications in regenerativemedicinetomodel,elucidate,andeventually treatvascular diseases. Here we report a robust two-step protocol that can be used to simultaneously generate large numbers of functional SMCs and ECs from a common proliferative vascular progenitor population via a two-dimensional culture system. We show here that coculturing hPSCs with OP9 cells in media supplemented with vascular endothelial growth factor, basic fibroblast growth factor, and bone morphogenetic protein 4 yields a higher percentage of CD31+CD34+ cells on day 8 of differentiation. Upon exposure to endothelial differentiationmedia andSMdifferentiationmedia, these vascular progenitors were able to differentiate andmature into functional endothelial cells and smoothmuscle cells, respectively. Furthermore, we were able to expand the intermediate population more than a billionfold to generate sufficientnumbers ofECsandSMCs inparallel forpotential therapeutic transplantations.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalStem Cells Translational Medicine
Volume3
Issue number1
DOIs
StatePublished - 2014

Keywords

  • Embryonic stem cells
  • Endothelial cells
  • Smooth muscle cells
  • Stem cell

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

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