Comprehensive characterization of interaction complexes of herpes simplex virus type 1 ICP22, UL3, UL4, and UL20.5

Junji Xing, Shuai Wang, Fusen Lin, Weiwei Pan, Chang Deng Hu, Chunfu Zheng

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

It has been reported that herpes simplex virus type 1 UL3, UL4, and UL20.5 proteins are localized to small, dense nuclear bodies together with ICP22 in infected cells. In the present study, we comprehensively characterized these interactions by subcellular colocalization, coimmunoprecipitation, and bimolecular fluorescence complementation assays. For the first time, it was demonstrated that both UL3 and UL20.5 are targeted to small, dense nuclear bodies by a direct interaction with ICP22, whereas UL4 colocalizes with ICP22 through its interaction with UL3 but not UL20.5 or ICP22. There was no detectable interaction between UL3 and UL20.5.

Original languageEnglish (US)
Pages (from-to)1881-1886
Number of pages6
JournalJournal of virology
Volume85
Issue number4
DOIs
StatePublished - Feb 2011

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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