Composite hydrogels containing polypyrrole as support membranes for amperometric enzyme biosensors

The amperometric enzyme biosensors were synthesized using composite hydrogels containing polypyrrole as support membranes. A simple two-step polymerization process was used to synthesize these biosensors. Ultra-violet polymerization of the hydrogel component was used to prepare electrode-supported composite films. This was followed by electrochemical polymerization of the pyrrole component within interstitial spaces of the pre-formed hydrogel networks. The long-term immobilization of enzymes and more favorable medium for the rapid movement of charge neutralizing ions was provided by the high water content of these materials.