Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing

B. Adinolfi; S. Carpi; A. Giannetti; P. Nieri; M. Pellegrino; G. Sotgiu; S. Tombelli; C. Trono; G. Varchi; F. Baldini

doi:10.1016/j.proeng.2014.11.645

Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing

B. Adinolfi, S. Carpi, A. Giannetti, P. Nieri, M. Pellegrino, G. Sotgiu, S. Tombelli, C. Trono, G. Varchi, F. Baldini

Research output: Contribution to journal › Conference article › peer-review

4 Scopus citations

Abstract

The design, implementation and characterization of complex nanostructures for intracellular mRNA monitoring constituted by a molecular beacon (MB), covalently immobilized onto polymethylmethacrylate (PMMA) fluorescent nanoparticles (NPs) are described. The MB molecule, adsorbed onto the nanoparticle for intracellular delivery, is capable to turn on the fluorescence emission of its fluorescent label, upon the interaction with a well-defined target mRNA. The mRNA specific for survivin has been chosen as target. A MB for survivin mRNA was characterized in vitro to verify its functionality. The functionality and specificity of the MB were also examined in living cells by transfection with a classical lipid agent, Lipofectamine. In addition, bear and MB-coated PMMA NPs were tested with adenocarcinomic human alveolar basal epithelial cells (A549) in terms of cell vitality and internalization.

Original language	English (US)
Pages (from-to)	751-754
Number of pages	4
Journal	Procedia Engineering
Volume	87
DOIs	https://doi.org/10.1016/j.proeng.2014.11.645
State	Published - 2014
Event	28th European Conference on Solid-State Transducers, EUROSENSORS 2014 - Brescia, Italy Duration: Sep 7 2014 → Sep 10 2014

Keywords

MRNA
Molecular beacon
PMMA nanoparticle
Survivin

ASJC Scopus subject areas

General Engineering

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10.1016/j.proeng.2014.11.645

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title = "Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing",

abstract = "The design, implementation and characterization of complex nanostructures for intracellular mRNA monitoring constituted by a molecular beacon (MB), covalently immobilized onto polymethylmethacrylate (PMMA) fluorescent nanoparticles (NPs) are described. The MB molecule, adsorbed onto the nanoparticle for intracellular delivery, is capable to turn on the fluorescence emission of its fluorescent label, upon the interaction with a well-defined target mRNA. The mRNA specific for survivin has been chosen as target. A MB for survivin mRNA was characterized in vitro to verify its functionality. The functionality and specificity of the MB were also examined in living cells by transfection with a classical lipid agent, Lipofectamine. In addition, bear and MB-coated PMMA NPs were tested with adenocarcinomic human alveolar basal epithelial cells (A549) in terms of cell vitality and internalization.",

keywords = "MRNA, Molecular beacon, PMMA nanoparticle, Survivin",

author = "B. Adinolfi and S. Carpi and A. Giannetti and P. Nieri and M. Pellegrino and G. Sotgiu and S. Tombelli and C. Trono and G. Varchi and F. Baldini",

note = "Funding Information: This work was supported by the regional project NANOCELL (PAR FAS REGIONE TOSCANA Linea 1.1.a.3) and by the national flagship project NANOMAX. Publisher Copyright: {\textcopyright} 2014 The Authors. Published by Elsevier Ltd.; 28th European Conference on Solid-State Transducers, EUROSENSORS 2014 ; Conference date: 07-09-2014 Through 10-09-2014",

year = "2014",

doi = "10.1016/j.proeng.2014.11.645",

language = "English (US)",

volume = "87",

pages = "751--754",

journal = "Procedia Engineering",

issn = "1877-7058",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing

AU - Adinolfi, B.

AU - Carpi, S.

AU - Giannetti, A.

AU - Nieri, P.

AU - Pellegrino, M.

AU - Sotgiu, G.

AU - Tombelli, S.

AU - Trono, C.

AU - Varchi, G.

AU - Baldini, F.

N1 - Funding Information: This work was supported by the regional project NANOCELL (PAR FAS REGIONE TOSCANA Linea 1.1.a.3) and by the national flagship project NANOMAX. Publisher Copyright: © 2014 The Authors. Published by Elsevier Ltd.

PY - 2014

Y1 - 2014

N2 - The design, implementation and characterization of complex nanostructures for intracellular mRNA monitoring constituted by a molecular beacon (MB), covalently immobilized onto polymethylmethacrylate (PMMA) fluorescent nanoparticles (NPs) are described. The MB molecule, adsorbed onto the nanoparticle for intracellular delivery, is capable to turn on the fluorescence emission of its fluorescent label, upon the interaction with a well-defined target mRNA. The mRNA specific for survivin has been chosen as target. A MB for survivin mRNA was characterized in vitro to verify its functionality. The functionality and specificity of the MB were also examined in living cells by transfection with a classical lipid agent, Lipofectamine. In addition, bear and MB-coated PMMA NPs were tested with adenocarcinomic human alveolar basal epithelial cells (A549) in terms of cell vitality and internalization.

AB - The design, implementation and characterization of complex nanostructures for intracellular mRNA monitoring constituted by a molecular beacon (MB), covalently immobilized onto polymethylmethacrylate (PMMA) fluorescent nanoparticles (NPs) are described. The MB molecule, adsorbed onto the nanoparticle for intracellular delivery, is capable to turn on the fluorescence emission of its fluorescent label, upon the interaction with a well-defined target mRNA. The mRNA specific for survivin has been chosen as target. A MB for survivin mRNA was characterized in vitro to verify its functionality. The functionality and specificity of the MB were also examined in living cells by transfection with a classical lipid agent, Lipofectamine. In addition, bear and MB-coated PMMA NPs were tested with adenocarcinomic human alveolar basal epithelial cells (A549) in terms of cell vitality and internalization.

KW - MRNA

KW - Molecular beacon

KW - PMMA nanoparticle

KW - Survivin

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U2 - 10.1016/j.proeng.2014.11.645

DO - 10.1016/j.proeng.2014.11.645

M3 - Conference article

AN - SCOPUS:84923319787

SN - 1877-7058

VL - 87

SP - 751

EP - 754

JO - Procedia Engineering

JF - Procedia Engineering

T2 - 28th European Conference on Solid-State Transducers, EUROSENSORS 2014

Y2 - 7 September 2014 through 10 September 2014

ER -

Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing

Abstract

Keywords

ASJC Scopus subject areas

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