Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing

B. Adinolfi, S. Carpi, A. Giannetti, P. Nieri, M. Pellegrino, G. Sotgiu, S. Tombelli, C. Trono, G. Varchi, F. Baldini

Research output: Contribution to journalConference article

3 Scopus citations

Abstract

The design, implementation and characterization of complex nanostructures for intracellular mRNA monitoring constituted by a molecular beacon (MB), covalently immobilized onto polymethylmethacrylate (PMMA) fluorescent nanoparticles (NPs) are described. The MB molecule, adsorbed onto the nanoparticle for intracellular delivery, is capable to turn on the fluorescence emission of its fluorescent label, upon the interaction with a well-defined target mRNA. The mRNA specific for survivin has been chosen as target. A MB for survivin mRNA was characterized in vitro to verify its functionality. The functionality and specificity of the MB were also examined in living cells by transfection with a classical lipid agent, Lipofectamine. In addition, bear and MB-coated PMMA NPs were tested with adenocarcinomic human alveolar basal epithelial cells (A549) in terms of cell vitality and internalization.

Original languageEnglish (US)
Pages (from-to)751-754
Number of pages4
JournalProcedia Engineering
Volume87
DOIs
StatePublished - 2014
Event28th European Conference on Solid-State Transducers, EUROSENSORS 2014 - Brescia, Italy
Duration: Sep 7 2014Sep 10 2014

Keywords

  • MRNA
  • Molecular beacon
  • PMMA nanoparticle
  • Survivin

ASJC Scopus subject areas

  • Engineering(all)

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