Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells

Ailiang Chen; Mingyong Luo; Guohua Yuan; Jian Yu; Tuo Deng; Liang Zhang; Yuxiang Zhou; Keith Mitchelson; Jing Cheng

doi:10.1007/s10529-008-9800-8

Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells

Ailiang Chen, Mingyong Luo, Guohua Yuan, Jian Yu, Tuo Deng, Liang Zhang, Yuxiang Zhou, Keith Mitchelson, Jing Cheng

Academic Institute

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

MicroRNAs (miRNAs) and mRNAs constitute an important part of gene regulatory networks, influencing diverse biological phenomena. To discover novel regulatory pathways during myeloid differentiation, we performed miRNA as well as mRNA expression profiling of in vitro-differentiating HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). The main findings were up-regulation of miR-146a/b, miR-21, miR-221, miR-222, miR-155, miR-26a and down-regulation of miR-199a*, miR-181c, miR-142-3p, miR-92. After integrating the miRNA and mRNA expression data into a Transcriptome Interaction Database by Molecule Annotation System (MAS) software, a number of differently expressed mRNAs were revealed as potential targets of these miRNAs.

Original language	English (US)
Pages (from-to)	2045-2052
Number of pages	8
Journal	Biotechnology Letters
Volume	30
Issue number	12
DOIs	https://doi.org/10.1007/s10529-008-9800-8
State	Published - Dec 2008

Keywords

Cell differentiation
HL-60
Microarray
MicroRNA
TPA

ASJC Scopus subject areas

Biotechnology

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10.1007/s10529-008-9800-8

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title = "Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells",

abstract = "MicroRNAs (miRNAs) and mRNAs constitute an important part of gene regulatory networks, influencing diverse biological phenomena. To discover novel regulatory pathways during myeloid differentiation, we performed miRNA as well as mRNA expression profiling of in vitro-differentiating HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). The main findings were up-regulation of miR-146a/b, miR-21, miR-221, miR-222, miR-155, miR-26a and down-regulation of miR-199a*, miR-181c, miR-142-3p, miR-92. After integrating the miRNA and mRNA expression data into a Transcriptome Interaction Database by Molecule Annotation System (MAS) software, a number of differently expressed mRNAs were revealed as potential targets of these miRNAs.",

keywords = "Cell differentiation, HL-60, Microarray, MicroRNA, TPA",

author = "Ailiang Chen and Mingyong Luo and Guohua Yuan and Jian Yu and Tuo Deng and Liang Zhang and Yuxiang Zhou and Keith Mitchelson and Jing Cheng",

note = "Funding Information: Acknowledgements This work was supported by a National Hi-Tech Program of China Grant (No. 2006AA020701).",

year = "2008",

month = dec,

doi = "10.1007/s10529-008-9800-8",

language = "English (US)",

volume = "30",

pages = "2045--2052",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

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TY - JOUR

T1 - Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells

AU - Chen, Ailiang

AU - Luo, Mingyong

AU - Yuan, Guohua

AU - Yu, Jian

AU - Deng, Tuo

AU - Zhang, Liang

AU - Zhou, Yuxiang

AU - Mitchelson, Keith

AU - Cheng, Jing

N1 - Funding Information: Acknowledgements This work was supported by a National Hi-Tech Program of China Grant (No. 2006AA020701).

PY - 2008/12

Y1 - 2008/12

N2 - MicroRNAs (miRNAs) and mRNAs constitute an important part of gene regulatory networks, influencing diverse biological phenomena. To discover novel regulatory pathways during myeloid differentiation, we performed miRNA as well as mRNA expression profiling of in vitro-differentiating HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). The main findings were up-regulation of miR-146a/b, miR-21, miR-221, miR-222, miR-155, miR-26a and down-regulation of miR-199a*, miR-181c, miR-142-3p, miR-92. After integrating the miRNA and mRNA expression data into a Transcriptome Interaction Database by Molecule Annotation System (MAS) software, a number of differently expressed mRNAs were revealed as potential targets of these miRNAs.

AB - MicroRNAs (miRNAs) and mRNAs constitute an important part of gene regulatory networks, influencing diverse biological phenomena. To discover novel regulatory pathways during myeloid differentiation, we performed miRNA as well as mRNA expression profiling of in vitro-differentiating HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). The main findings were up-regulation of miR-146a/b, miR-21, miR-221, miR-222, miR-155, miR-26a and down-regulation of miR-199a*, miR-181c, miR-142-3p, miR-92. After integrating the miRNA and mRNA expression data into a Transcriptome Interaction Database by Molecule Annotation System (MAS) software, a number of differently expressed mRNAs were revealed as potential targets of these miRNAs.

KW - Cell differentiation

KW - HL-60

KW - Microarray

KW - MicroRNA

KW - TPA

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UR - http://www.scopus.com/inward/citedby.url?scp=54849412067&partnerID=8YFLogxK

U2 - 10.1007/s10529-008-9800-8

DO - 10.1007/s10529-008-9800-8

M3 - Article

C2 - 18648749

AN - SCOPUS:54849412067

SN - 0141-5492

VL - 30

SP - 2045

EP - 2052

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 12

ER -

Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells

Abstract

Keywords

ASJC Scopus subject areas

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