Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

Alina J. Andersen; Barbara Windschiegl; Sibel Ilbasmis-Tamer; Ismail T. Degim; Alan Christy Hunter; Thomas L. Andresen; Seyed Moein Moghimi

doi:10.1016/j.nano.2013.01.011

Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

Alina J. Andersen, Barbara Windschiegl, Sibel Ilbasmis-Tamer, Ismail T. Degim, Alan Christy Hunter, Thomas L. Andresen, Seyed Moein Moghimi

Houston Methodist

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Carboxylated (4%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)₁₀₀₀ (PEG₁₀₀₀), PEG₁₅₀₀ and PEG₄₀₀₀ with a PEG loading of approximately 11% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG_{2000(or 5000)}-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies.

Original language	English (US)
Pages (from-to)	469-473
Number of pages	5
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/j.nano.2013.01.011
State	Published - May 2013

Keywords

Atomic force microscope
Carbon nanotubes
Complement system
Innate immunity
Poly(ethylene glycol)

ASJC Scopus subject areas

Molecular Medicine
Bioengineering
Biomedical Engineering
General Materials Science
Medicine (miscellaneous)
Pharmaceutical Science

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10.1016/j.nano.2013.01.011

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title = "Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density",

abstract = "Carboxylated (4\%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)1000 (PEG1000), PEG1500 and PEG4000 with a PEG loading of approximately 11\% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG2000(or 5000)-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies.",

keywords = "Atomic force microscope, Carbon nanotubes, Complement system, Innate immunity, Poly(ethylene glycol)",

author = "Andersen, \{Alina J.\} and Barbara Windschiegl and Sibel Ilbasmis-Tamer and Degim, \{Ismail T.\} and Hunter, \{Alan Christy\} and Andresen, \{Thomas L.\} and Moghimi, \{Seyed Moein\}",

note = "Funding Information: Financial support by the Danish Agency for Science, Technology and Innovation (Det Strategiske Forskningsr{\aa}d) , reference 09-065746 (to SMM), is gratefully acknowledged. AJA is a recipient of PhD scholarship award from the University of Copenhagen. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",

year = "2013",

month = may,

doi = "10.1016/j.nano.2013.01.011",

language = "English (US)",

volume = "9",

pages = "469--473",

journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",

issn = "1549-9634",

publisher = "Elsevier",

number = "4",

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TY - JOUR

T1 - Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

AU - Andersen, Alina J.

AU - Windschiegl, Barbara

AU - Ilbasmis-Tamer, Sibel

AU - Degim, Ismail T.

AU - Hunter, Alan Christy

AU - Andresen, Thomas L.

AU - Moghimi, Seyed Moein

N1 - Funding Information: Financial support by the Danish Agency for Science, Technology and Innovation (Det Strategiske Forskningsråd) , reference 09-065746 (to SMM), is gratefully acknowledged. AJA is a recipient of PhD scholarship award from the University of Copenhagen. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2013/5

Y1 - 2013/5

N2 - Carboxylated (4%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)1000 (PEG1000), PEG1500 and PEG4000 with a PEG loading of approximately 11% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG2000(or 5000)-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies.

AB - Carboxylated (4%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)1000 (PEG1000), PEG1500 and PEG4000 with a PEG loading of approximately 11% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG2000(or 5000)-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies.

KW - Atomic force microscope

KW - Carbon nanotubes

KW - Complement system

KW - Innate immunity

KW - Poly(ethylene glycol)

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AN - SCOPUS:84876719486

SN - 1549-9634

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SP - 469

EP - 473

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

IS - 4

ER -

Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

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Keywords

ASJC Scopus subject areas

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