Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

Lydia Kisley; Ujwal Patil; Sagar Dhamane; Katerina Kourentzi; Lawrence J. Tauzin; Richard C. Willson; Christy F. Landes

doi:10.1039/c7an00701a

Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

Lydia Kisley, Ujwal Patil, Sagar Dhamane, Katerina Kourentzi, Lawrence J. Tauzin, Richard C. Willson, Christy F. Landes

Houston Methodist

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We use single molecule spectroscopy to study a multicomponent, competitive protein adsorption system. Fluorescently-labeled α-lactalbumin proteins are super-resolved adsorbing to cationic anion-exchange ligands in the presence of a competitor, insulin. We find that the competitor reduces the number of binding events by blocking ligands throughout the observed measurement time while the single-site adsorption kinetics are unchanged.

Original language	English (US)
Pages (from-to)	3127-3131
Number of pages	5
Journal	Analyst
Volume	142
Issue number	17
DOIs	https://doi.org/10.1039/c7an00701a
State	Published - Sep 7 2017

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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AU - Tauzin, Lawrence J.

AU - Willson, Richard C.

AU - Landes, Christy F.

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Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

Abstract

ASJC Scopus subject areas

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