Abstract
We use single molecule spectroscopy to study a multicomponent, competitive protein adsorption system. Fluorescently-labeled α-lactalbumin proteins are super-resolved adsorbing to cationic anion-exchange ligands in the presence of a competitor, insulin. We find that the competitor reduces the number of binding events by blocking ligands throughout the observed measurement time while the single-site adsorption kinetics are unchanged.
Original language | English (US) |
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Pages (from-to) | 3127-3131 |
Number of pages | 5 |
Journal | Analyst |
Volume | 142 |
Issue number | 17 |
DOIs | |
State | Published - Sep 7 2017 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry