Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

Lydia Kisley, Ujwal Patil, Sagar Dhamane, Katerina Kourentzi, Lawrence J. Tauzin, Richard C. Willson, Christy F. Landes

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We use single molecule spectroscopy to study a multicomponent, competitive protein adsorption system. Fluorescently-labeled α-lactalbumin proteins are super-resolved adsorbing to cationic anion-exchange ligands in the presence of a competitor, insulin. We find that the competitor reduces the number of binding events by blocking ligands throughout the observed measurement time while the single-site adsorption kinetics are unchanged.

Original languageEnglish (US)
Pages (from-to)3127-3131
Number of pages5
JournalAnalyst
Volume142
Issue number17
DOIs
StatePublished - Sep 7 2017

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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