Competitive binding of 7-substituted-2,3-dichlorodibenzo-p-dioxins with human placental Ah receptor-A QSAR analysis

Cheryl L. Golas, Rebecca D. Prokipcak, Allan B. Okey, David K. Manchester, Stephen Safe, Fujita Toshio

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The competitive binding affinities of thirteen 7-substituted-2,3-dichlorodibenzo-p-dioxins to the human placental cytosolic aryl hydrocarbon (Ah) receptor were determined using [3H]2,3,7,8tetrachlorodibenzo-p-dioxin as the radioligand. Multiple parameter linear regression analysis of the competitive binding ec50 values for these compounds gave the following equation: pec50(M) = 6.246 + 1.632 π - 1.764 σ0m + 1.282 HB where π, σ0m and HB are the physiochemical parameters for substituent lipophilicity, meta-directing electronegativity, and hydrogen bonding capacity respectively. The 7-t-butyl- and 7-phenyl-2,3-dichlorodibenzo-p-dioxins were treated as outliers for the derivation of this equation, and these results suggest that only substituents with van der Waals' volumes < 40 cm3/mol were accommodated in the receptor binding site. The equations previously derived from the binding of the 7-substituted-2,3-dichlorodibenzo-p-dioxins to the rat, mouse, guinea pig and hamster hepatic cytosolic receptor were different than the correlation obtained using human placental receptor and provide further evidence for the interspecies differences in the molecular and binding properties of the Ah receptor protein.

Original languageEnglish (US)
Pages (from-to)737-741
Number of pages5
JournalBiochemical pharmacology
Volume40
Issue number4
DOIs
StatePublished - Aug 15 1990

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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