Comparison of three methods for measuring factor VIII levels in plasma

Wayne L. Chandler, Chris Ferrell, Joo Lee, Theingi Tun, Hien Kha

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage clot-based and chromogenic factor VIII assays (r 2 = 0.934), and the lowest correlation was between the 1-stage clot-based and antigenic factor VIII assays (r2 = 0.821). The presence of heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors in the sample resulted in major interference in the 1-stage clot-based assay but not the chromogenic or antigenic factor VIII assays. Overall, the chromogenic factor VIII activity assay was the optimal method, showing good precision, the best overall correlation with other assays, and no interference from heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume120
Issue number1
DOIs
StatePublished - Jul 1 2003

Keywords

  • Activity assay
  • Chromogenic assay
  • Factor VIII
  • Immunoassay

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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