TY - JOUR
T1 - Comparison of three methods for measuring factor VIII levels in plasma
AU - Chandler, Wayne L.
AU - Ferrell, Chris
AU - Lee, Joo
AU - Tun, Theingi
AU - Kha, Hien
PY - 2003/7/1
Y1 - 2003/7/1
N2 - We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage clot-based and chromogenic factor VIII assays (r 2 = 0.934), and the lowest correlation was between the 1-stage clot-based and antigenic factor VIII assays (r2 = 0.821). The presence of heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors in the sample resulted in major interference in the 1-stage clot-based assay but not the chromogenic or antigenic factor VIII assays. Overall, the chromogenic factor VIII activity assay was the optimal method, showing good precision, the best overall correlation with other assays, and no interference from heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors.
AB - We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage clot-based and chromogenic factor VIII assays (r 2 = 0.934), and the lowest correlation was between the 1-stage clot-based and antigenic factor VIII assays (r2 = 0.821). The presence of heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors in the sample resulted in major interference in the 1-stage clot-based assay but not the chromogenic or antigenic factor VIII assays. Overall, the chromogenic factor VIII activity assay was the optimal method, showing good precision, the best overall correlation with other assays, and no interference from heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors.
KW - Activity assay
KW - Chromogenic assay
KW - Factor VIII
KW - Immunoassay
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U2 - 10.1309/C8T8-YNB4-G3W4-5PRF
DO - 10.1309/C8T8-YNB4-G3W4-5PRF
M3 - Article
C2 - 12866370
SN - 0002-9173
VL - 120
SP - 34
EP - 39
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 1
ER -