Comparison of Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 2.0 (CAP/CTM v2.0) with other real-time PCR assays in HIV-1 monitoring and follow-up of low-level viral loads

Christina M. Wojewoda, Timothy Spahlinger, Marlene Louise Harmon, Brian Schnellinger, Qing Li, Corazon Dejelo, Christine Schmotzer, Lan Zhou

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Viral load monitoring of HIV-1 has become standard of care in HIV-1 positive patients. In this study, we evaluated the performance characteristics of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 2.0 (CAP/CTM v2.0) in comparison with Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 1.0 (CAP/CTM v1.0) and Abbott RealTime HIV-1 assay (m2000), with special emphasis on the quantitation of clinically controversial low-level viral loads. The performance characteristics of CAP/CTM v2.0 were confirmed by the validation study. All three assays performed comparably, with Abbott m2000 showing slightly decreased sensitivity for detection of viral loads close to the lower limit of quantitation. Follow-up of patients with low-level viral loads revealed that some of those represent single viral blips; however, a significant portion of these patients have intermittent or persistent low-positive viremia. We conclude that CAP/CTM v2.0 is an accurate and reliable assay for HIV-1 viral load monitoring.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of Virological Methods
Volume187
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • HIV-1
  • Real-time PCR
  • Viral blip
  • Viral load

ASJC Scopus subject areas

  • Virology

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