Comparison of hplc, tlc and minicolumn for measuring the radiochemical purity of 99tcm-q12

Meixiang Max Yu, K. A. BergstrÖM, J. T. Kuikka, J. Yang, E. Vanninen

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


TechneScan Q12® (99Tc(m)-Q12) is a new agent for clinical myocardial perfusion imaging. A product with high radiochemical purity is essential for high-quality imaging. We compared three methods of radiochemical purity analysis for 99Tc(m)-Q12: thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and minicolumn. Thin-layer chromatography resulted in lower purity (87 ± 8%) than the minicolumn method (aluminium oxide minicolumn, ethanol) (95 ± 4%). The HPLC method resulted in the lowest purity (79 ± 11%). The main impurity was a polar compound, but three further impurities were found using HPLC. Using HPLC, we found the percentage of the parent compound (99Tc(m)-Q12) in plasma at 40-90 min post-injection to be 19.3 ± 6.2%. We suggest that gradient HPLC is the most effective method for the analysis of the radiochemical purity of 99Tc(m)-Q12, and that it can be used to determine the concentration of 99Tc(m)-Q12 in plasma.

Original languageEnglish (US)
Pages (from-to)483-489
Number of pages7
JournalNuclear medicine communications
Issue number5
StatePublished - Jan 1 1998

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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