Abstract
TechneScan Q12® (99Tc(m)-Q12) is a new agent for clinical myocardial perfusion imaging. A product with high radiochemical purity is essential for high-quality imaging. We compared three methods of radiochemical purity analysis for 99Tc(m)-Q12: thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and minicolumn. Thin-layer chromatography resulted in lower purity (87 ± 8%) than the minicolumn method (aluminium oxide minicolumn, ethanol) (95 ± 4%). The HPLC method resulted in the lowest purity (79 ± 11%). The main impurity was a polar compound, but three further impurities were found using HPLC. Using HPLC, we found the percentage of the parent compound (99Tc(m)-Q12) in plasma at 40-90 min post-injection to be 19.3 ± 6.2%. We suggest that gradient HPLC is the most effective method for the analysis of the radiochemical purity of 99Tc(m)-Q12, and that it can be used to determine the concentration of 99Tc(m)-Q12 in plasma.
Original language | English (US) |
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Pages (from-to) | 483-489 |
Number of pages | 7 |
Journal | Nuclear medicine communications |
Volume | 19 |
Issue number | 5 |
DOIs | |
State | Published - Jan 1 1998 |
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging