Comparison of HPLC and GC-MS for measurement of cocaine and metabolites in human urine

Diane L. Phillips, Ian R. Tebbett, Roger L. Bertholf

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


High-performance liquid chromatography (HPLC) was compared with gas chromatography-mass spectrometry (GC-MS) for quantitation of cocaine, benzoylecgonine, norcocaine, and cocaethylene in urine. Calibration standards were prepared in human urine, and bupivacaine was added as the internal standard for quantitation. After solid-phase extraction, the reconstituted samples were divided into aliquots for analysis by HPLC and GC-MS. The analytical performance of the two methods were compared with regard to sensitivity, precision, and dynamic range. Results of GC-MS and HPLC analyses of nine urine specimens previously confirmed as positive for benzoylecgonine were compared. Analytical results by HPLC were comparable to GC-MS. Therefore, for many laboratories, HPLC is a useful alternative to GC- MS for measuring cocaine and metabolites in urine.

Original languageEnglish (US)
Pages (from-to)305-308
Number of pages4
JournalJournal of Analytical Toxicology
Issue number5
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety


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