Comparison of DNA fragmentation and color thresholding for objective quantitation of apoptotic cells

D. R. Plymale, D. S.N. Tang, C. D. Fermin, D. E. Lewis, D. S. Martin, R. F. Garry, P. W. Hawkes, D. Dinsdale, E. Falcieri

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Apoptosis is a process of cell death characterized by distinctive morphological changes and fragmentation of cellular DNA. Using video imaging and color thresholding techniques, we objectively quantitated the number of cultured CD4+ T-lymphoblastoid cells (HUT78 cells, RH9 subclone) displaying morphological signs of apoptosis before and after exposure to γ-irradiation. The numbers of apoptotic cells measured by objective video imaging techniques were compared to numbers of apoptotic cells measured in the same samples by sensitive apoptotic assays that quantitate DNA fragmentation. DNA fragmentation assays gave consistently higher values compared with the video imaging assays that measured morphological changes associated with apoptosis. These results suggest that substantial DNA fragmentation can precede or occur in the absence of the morphological changes which are associated with apoptosis in γ-irradiated RH9 cells.

Original languageEnglish (US)
Pages (from-to)833-842
Number of pages10
JournalScanning Microscopy
Volume9
Issue number3
StatePublished - 1995

Keywords

  • Apoptosis
  • DNA fragmentation
  • color thresholding
  • human-immunodeficiency virus
  • γ-irradiation

ASJC Scopus subject areas

  • Instrumentation

Fingerprint Dive into the research topics of 'Comparison of DNA fragmentation and color thresholding for objective quantitation of apoptotic cells'. Together they form a unique fingerprint.

Cite this