Comparison of clot-based vs chromogenic factor Xa procoagulant phospholipid activity assays

Sandra D. Bohling, Monica B. Pagano, Maria R. Stitzel, Chris Ferrell, Wandy Yeung, Wayne L. Chandler

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We compared 2 commercial plasma procoagulant phospholipid activity (PPA) assays, chromogenic, using bound annexin V to capture phosphatidylserine- containing microparticles, and clot-based. In both, anionic phospholipids accelerated activation of prothrombin by factor Xa. PPA levels were lower in the chromogenic vs the clot-based assay, with poor correlation between methods: normal samples, mean ± SD, 27 ± 17 vs 590 ± 414 nmol/L (n = 24; r 2 = 0.29) and patient samples, mean ± SD, 45 ± 44 vs 401 ± 330 nmol/L (n = 51; r 2 = 0.26). Recovery of phosphatidylserine added to normal, heparinized, and warfarin plasma samples averaged 109% ± 39% using the chromogenic assay but was higher and more varied (mean ± SD, 176% ± 59%) in the clot-based assay. Lupus anticoagulants caused low recovery in both assays. Removal of microparticles by 0.22-μm filtration reduced PPA by 91% in the clot-based and 65% in the chromogenic assay. The clot-based assay showed higher correlation (r 2 = 0.82 vs 0.23) with flow cytometric platelet microparticle counts. The 2 assays measure different aspects of PPA in plasma, with the chromogenic assay primarily measuring smaller microparticles.

Original languageEnglish (US)
Pages (from-to)185-192
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume137
Issue number2
DOIs
StatePublished - Feb 2012

Keywords

  • Chromogenic factor Xa procoagulant phospholipid activity assay
  • Clot-based assay
  • Coagulation
  • Phospholipid
  • Procoagulant phospholipid activity assay

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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