Comparative studies on plasma low density lipoproteins from pig and man

Richard L. Jackson, O. David Taunton, Ramon Segura, Joseph G. Gallagher, Henry F. Hoff, Antonio M. Gotto

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

1. 1. Low density lipoproteins (LDL) were isolated from pig plasma by ultracentrifugal flotation in KBr between densities 1·020 and 1·060 g/ml (LDL1) and between 1·060 and 1·090 g/ml (LDL2). 2. 2. The chemical, immunological and physical properties of LDL1 and LDL2 were compared with each other and with human LDL. 3. 3. LDL1 and LDL2 were delipidated and fractionated by chromatography on Sephadex G-150 in 0·1 M Tris-HC1, pH 8·0, containing 2 mM sodium decyl sulfate. ApoLDL1 and apoLDL2 were immunochemically identical, had only minor differences of amino acid composition and had indistinguishable circular dichroic spectra. The apoproteins were very similar in amino acid composition and circular dichroic spectra to human apoLDL. 4. 4. Our findings strongly suggest that the apoproteins from pig LDL1 and LDL2 are identical and that this protein is similar but not identical to apoLDL from man.

Original languageEnglish (US)
Pages (from-to)245-253
Number of pages9
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume53
Issue number2
DOIs
StatePublished - 1976

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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