Comparative studies on plasma low density lipoproteins from pig and man

Richard L. Jackson; O. David Taunton; Ramon Segura; Joseph G. Gallagher; Henry F. Hoff; Antonio M. Gotto

doi:10.1016/0305-0491(76)90043-2

Comparative studies on plasma low density lipoproteins from pig and man

Richard L. Jackson, O. David Taunton, Ramon Segura, Joseph G. Gallagher, Henry F. Hoff, Antonio M. Gotto

Research output: Contribution to journal › Article › peer-review

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Abstract

1. 1. Low density lipoproteins (LDL) were isolated from pig plasma by ultracentrifugal flotation in KBr between densities 1·020 and 1·060 g/ml (LDL₁) and between 1·060 and 1·090 g/ml (LDL₂). 2. 2. The chemical, immunological and physical properties of LDL₁ and LDL₂ were compared with each other and with human LDL. 3. 3. LDL₁ and LDL₂ were delipidated and fractionated by chromatography on Sephadex G-150 in 0·1 M Tris-HC1, pH 8·0, containing 2 mM sodium decyl sulfate. ApoLDL₁ and apoLDL₂ were immunochemically identical, had only minor differences of amino acid composition and had indistinguishable circular dichroic spectra. The apoproteins were very similar in amino acid composition and circular dichroic spectra to human apoLDL. 4. 4. Our findings strongly suggest that the apoproteins from pig LDL₁ and LDL₂ are identical and that this protein is similar but not identical to apoLDL from man.

Original language	English (US)
Pages (from-to)	245-253
Number of pages	9
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	53
Issue number	2
DOIs	https://doi.org/10.1016/0305-0491(76)90043-2
State	Published - 1976

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

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@article{a627709f09514905a05d4b3d50f5a242,

title = "Comparative studies on plasma low density lipoproteins from pig and man",

abstract = "1. 1. Low density lipoproteins (LDL) were isolated from pig plasma by ultracentrifugal flotation in KBr between densities 1·020 and 1·060 g/ml (LDL1) and between 1·060 and 1·090 g/ml (LDL2). 2. 2. The chemical, immunological and physical properties of LDL1 and LDL2 were compared with each other and with human LDL. 3. 3. LDL1 and LDL2 were delipidated and fractionated by chromatography on Sephadex G-150 in 0·1 M Tris-HC1, pH 8·0, containing 2 mM sodium decyl sulfate. ApoLDL1 and apoLDL2 were immunochemically identical, had only minor differences of amino acid composition and had indistinguishable circular dichroic spectra. The apoproteins were very similar in amino acid composition and circular dichroic spectra to human apoLDL. 4. 4. Our findings strongly suggest that the apoproteins from pig LDL1 and LDL2 are identical and that this protein is similar but not identical to apoLDL from man.",

author = "Jackson, {Richard L.} and {David Taunton}, O. and Ramon Segura and Gallagher, {Joseph G.} and Hoff, {Henry F.} and Gotto, {Antonio M.}",

note = "Funding Information: lent technicala ssistance.W e thank William R. Voss, LOWRY O. H., ROSEBROUGHN . J., FARR A. L. & RANDALL pp. 181-274W. iley lntersciencNe,e w York. D.V.M., for his help in bleedinga nd housing thpei gs. We R. J. (19511P roteinm easuremenwtsit h the Folin phenol acknowledgteh ee xcellenats sistancoe f Ms. DebbieM ason reagentJ.. biol. Chem1. 93,2 65 275. in the preparation tohfe manuscriptT.h is work was sup-Ltlx S. E., HIRZ R., SHRAGERR . I. & GOTTOA . M. (1972) portedi n part by Health,E ducation anWd elfareG rants The influenceo f lipid on the conformatioonf human HL 16544-01M ET; by contractN o. NIH 71-2156u nder No. HL 14194H, L 0534-1451b; y NIH grant No. 1 R01 plasmah igh densityl ipoproteinsJ.. biol. Chem.2 47, Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1976",

doi = "10.1016/0305-0491(76)90043-2",

language = "English (US)",

volume = "53",

pages = "245--253",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "0305-0491",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Comparative studies on plasma low density lipoproteins from pig and man

AU - Jackson, Richard L.

AU - David Taunton, O.

AU - Segura, Ramon

AU - Gallagher, Joseph G.

AU - Hoff, Henry F.

AU - Gotto, Antonio M.

N1 - Funding Information: lent technicala ssistance.W e thank William R. Voss, LOWRY O. H., ROSEBROUGHN . J., FARR A. L. & RANDALL pp. 181-274W. iley lntersciencNe,e w York. D.V.M., for his help in bleedinga nd housing thpei gs. We R. J. (19511P roteinm easuremenwtsit h the Folin phenol acknowledgteh ee xcellenats sistancoe f Ms. DebbieM ason reagentJ.. biol. Chem1. 93,2 65 275. in the preparation tohfe manuscriptT.h is work was sup-Ltlx S. E., HIRZ R., SHRAGERR . I. & GOTTOA . M. (1972) portedi n part by Health,E ducation anWd elfareG rants The influenceo f lipid on the conformatioonf human HL 16544-01M ET; by contractN o. NIH 71-2156u nder No. HL 14194H, L 0534-1451b; y NIH grant No. 1 R01 plasmah igh densityl ipoproteinsJ.. biol. Chem.2 47, Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1976

Y1 - 1976

N2 - 1. 1. Low density lipoproteins (LDL) were isolated from pig plasma by ultracentrifugal flotation in KBr between densities 1·020 and 1·060 g/ml (LDL1) and between 1·060 and 1·090 g/ml (LDL2). 2. 2. The chemical, immunological and physical properties of LDL1 and LDL2 were compared with each other and with human LDL. 3. 3. LDL1 and LDL2 were delipidated and fractionated by chromatography on Sephadex G-150 in 0·1 M Tris-HC1, pH 8·0, containing 2 mM sodium decyl sulfate. ApoLDL1 and apoLDL2 were immunochemically identical, had only minor differences of amino acid composition and had indistinguishable circular dichroic spectra. The apoproteins were very similar in amino acid composition and circular dichroic spectra to human apoLDL. 4. 4. Our findings strongly suggest that the apoproteins from pig LDL1 and LDL2 are identical and that this protein is similar but not identical to apoLDL from man.

AB - 1. 1. Low density lipoproteins (LDL) were isolated from pig plasma by ultracentrifugal flotation in KBr between densities 1·020 and 1·060 g/ml (LDL1) and between 1·060 and 1·090 g/ml (LDL2). 2. 2. The chemical, immunological and physical properties of LDL1 and LDL2 were compared with each other and with human LDL. 3. 3. LDL1 and LDL2 were delipidated and fractionated by chromatography on Sephadex G-150 in 0·1 M Tris-HC1, pH 8·0, containing 2 mM sodium decyl sulfate. ApoLDL1 and apoLDL2 were immunochemically identical, had only minor differences of amino acid composition and had indistinguishable circular dichroic spectra. The apoproteins were very similar in amino acid composition and circular dichroic spectra to human apoLDL. 4. 4. Our findings strongly suggest that the apoproteins from pig LDL1 and LDL2 are identical and that this protein is similar but not identical to apoLDL from man.

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U2 - 10.1016/0305-0491(76)90043-2

DO - 10.1016/0305-0491(76)90043-2

M3 - Article

C2 - 175991

AN - SCOPUS:0017251287

VL - 53

SP - 245

EP - 253

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

SN - 0305-0491

IS - 2

ER -

Comparative studies on plasma low density lipoproteins from pig and man

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