TY - JOUR
T1 - Comparative physicochemical and biological properties of two strains of Kilham rat virus, a non-defective parvovirus
AU - Mitra, S.
AU - Snyder, C. E.
AU - Bates, R. C.
AU - Banerjee, P. T.
PY - 1982
Y1 - 1982
N2 - Two antigenically indistinguishable strains, 171 and 308, of Kilham rat virus (KRV) have distinct host ranges and contain capsid proteins of identical size, but with different isoelectric points. The single-stranded DNA genomes of the viruses are also the same size but appear to have different secondary and tertiary structures. The genomes of the two strains have nearly identical cleavage maps for 11 restriction endonucleases, except of differences in five restriction sites in the region bracketed by 0.63 to 0.90 map units (from the 3' ends of the virus strands). However, there is a lack of extended heterology in the nucleotide sequence of the two virus genomes, as judged by electron microscopic analysis of the heteroduplex of the two virus DNAs. This suggests that very subtle differences in the sequences of the genome, and possibly of the capsid proteins, may play a role in the host specificity without affecting the antigenic similarity of KRV strains.
AB - Two antigenically indistinguishable strains, 171 and 308, of Kilham rat virus (KRV) have distinct host ranges and contain capsid proteins of identical size, but with different isoelectric points. The single-stranded DNA genomes of the viruses are also the same size but appear to have different secondary and tertiary structures. The genomes of the two strains have nearly identical cleavage maps for 11 restriction endonucleases, except of differences in five restriction sites in the region bracketed by 0.63 to 0.90 map units (from the 3' ends of the virus strands). However, there is a lack of extended heterology in the nucleotide sequence of the two virus genomes, as judged by electron microscopic analysis of the heteroduplex of the two virus DNAs. This suggests that very subtle differences in the sequences of the genome, and possibly of the capsid proteins, may play a role in the host specificity without affecting the antigenic similarity of KRV strains.
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U2 - 10.1099/0022-1317-61-1-43
DO - 10.1099/0022-1317-61-1-43
M3 - Article
C2 - 6288851
AN - SCOPUS:0020315352
SN - 0022-1317
VL - 61
SP - 43
EP - 54
JO - Journal of General Virology
JF - Journal of General Virology
IS - 1
ER -