Comparative effects of torasemide and furosemide on gap junction proteins and cardiac fibrosis in a rat model of dilated cardiomyopathy

Kenichi Watanabe, Remya Sreedhar, Rajarajan A. Thandavarayan, Vengadeshprabhu Karuppagounder, Vijayasree V. Giridharan, Shanish Antony, Meilei Harima, Masahiko Nakamura, Kenji Suzuki, Hiroshi Suzuki, Hirohito Sone, Somasundaram Arumugam

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Cardiac fibrosis is the major hallmark of adverse cardiac remodeling in chronic heart failure (CHF) and its therapeutic targeting might help against cardiac dysfunction during chronic conditions. Diuretic agents are potentially useful in these cases, but their effects on the cardiac fibrosis pathogenesis are yet to be identified. This study was designed to identify and compare the effects of diuretic drugs torasemide and furosemide on cardiac fibrosis in a rat model of dilated cardiomyopathy induced by porcine cardiac myosin mediated experimental autoimmune myocarditis. Gap junction proteins, connexin-43 and N-cadherin, expressions were downregulated in the hearts of CHF rats, while torasemide treatment has upregulated their expression. Western blotting and immunohistochemical analysis for various cardiac fibrosis related proteins as well as histopathological studies have shown that both drugs have potential anti-fibrotic effects. Among them, torasemide has superior efficacy in offering protection against adverse cardiac remodeling in the selected rat model of dilated cardiomyopathy. In conclusion, torasemide treatment has potential anti-fibrotic effect in the hearts of CHF rats, possibly via improving the gap junction proteins expression and thereby improving the cell–cell interaction in the heart.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalBioFactors
Volume43
Issue number2
DOIs
StatePublished - Mar 1 2017

Keywords

  • cardiac fibrosis
  • connexin-43
  • furosemide
  • gap junction
  • torasemide

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

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