Common sites of retroviral integration in mouse hematopoietic tumors identified by high-throughput, single nucleotide polymorphism-based mapping and bacterial artificial chromosome hybridization

Haifa Shen, Takeshi Suzuki, David J. Munroe, Claudia Stewart, Lynn Rasmussen, Debra J. Gilbert, Nancy A. Jenkins, Neal G. Copeland

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Retroviral insertional mutagenesis in mouse hematopoietic tumors provides a powerful cancer gene discovery tool. Here, we describe a high-throughput, single nucleotide polymorphism (SNP)-based method, for mapping retroviral integration sites cloned from mouse tumors, and a bacterial artificial chromosome (BAC) hybridization method, for localizing these retroviral integration sites to common sites of retroviral integration (CISs). Several new CISs were identified, including one CIS that mapped near Notch1, a gene that has been causally associated with human T-cell tumors. This mapping method is applicable to many different species, including ones where few genetic markers and little genomic sequence information are available. It can also be used to map endogenous proviruses.

Original languageEnglish (US)
Pages (from-to)1584-1588
Number of pages5
JournalJournal of virology
Volume77
Issue number2
DOIs
StatePublished - Jan 2003

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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