Combining nuclear magnetic resonance spectroscopy and mass spectrometry in biomarker discovery

G. A.Nagana Gowda, Omkar B. Ijare, Narasimhamurthy Shanaiah

Research output: Contribution to journalReview article

7 Scopus citations

Abstract

Metabolic profiling of biological specimens is emerging as a promising approach for discovering specific biomarkers in the diagnosis of a number of diseases. Amongst many analytical techniques, nuclear magnetic resonance spectroscopy and mass spectrometry are the most information-rich tools that enable high-throughput and global analysis of hundreds of metabolites in a single step. Although only one of the two techniques is utilized in a majority of metabolomics applications, there is a growing interest in combining the data from the two methods to effectively unravel the mammoth complexity of biological samples. In this article, current developments in nuclear magnetic resonance, mass spectrometry and multivariate statistical analysis methods are described. While some general applications that utilize the combination of the two analytical methods are presented briefly, the emphasis is laid on the recent applications of nuclear magnetic resonance and mass spectrometry methods in the studies of hepatopancreatobiliary and gastrointestinal malignancies.

Original languageEnglish (US)
Pages (from-to)307-322
Number of pages16
JournalBiomarkers in Medicine
Volume3
Issue number3
DOIs
StatePublished - Aug 6 2009

Keywords

  • Cancer
  • Cholangiocarcinoma
  • Colon cancer
  • Gallbladder cancer
  • Gastric cancer
  • Gastrointestinal
  • Hepatobiliary diseases
  • Hepatocellular carcinoma
  • Mass spectrometry
  • Metabolic profiling
  • Metabolomics
  • Multivariate statistical analysis
  • NMR spectroscopy
  • Pancreatic cancer

ASJC Scopus subject areas

  • Drug Discovery
  • Clinical Biochemistry
  • Biochemistry, medical

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