Colocalization of peptide and glucocorticoid receptor immunoreactivities in rat central amygdaloid nucleus

Jari Honkaniemi, Markku Pello-Huikko, Leena Rechardt, Jorma Isola, Antti Lammi, Kjell Fuxe, Jan-Ake Gustafsson, Anti Charlotte Wikström, Tomas Hökfelt

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

The central amygdaloid nucleus (ACe) is part of the amygdaloid complex that participates in adrenocorticotrophin secretion, stress-related reactions and behavioral functions. The ACe contains numerous glucocorticoid receptor (GR)-immunoreactive (IR) neurons, and in addition it has been shown to contain several neuropeptide-TR somata and nerve terminals. In order to study the relationship between the GR- and neuropeptide-IR structures we mapped the distribution of GR-like immunoreactivity (LT) in amygdaloid complex and colocalized the neuropeptide- and GR-LIs in the ACe. In the amygdaloid complex the central, medial and cortical nuclei contained a high number of GR-IR neurons, whereas a moderate number of GR-TR neurons were observed in the basolateral and basomedial nuclei. Only a few GR-IR neurons were seen in the lateral nucleus. In the ACe, the majority of corticotrophin-releasing factor (CRF)-, met-enkephalin (met-ENK)-, neurotensin (NT)- and somatostatin (SOM)-IR neurons contained also GR-IR. About half of the substance P(SP)-IR neurons were seen to contain GR-IR, whereas only some of the few vasoactive intestinal polypeptide and cholecystokinin-IR neurons showed GR-LI. Nerve terminals containing calcitonin gene-related peptide and the above mentioned peptides were seen in close contact with the GR-IR neurons. These results suggest that the glucocorticoids may modulate directly the neurotransmitter synthesis of the CRF-, met-ENK, NT-, SOM- and SP-IR cells in the ACe.

Original languageEnglish (US)
Pages (from-to)451-459
Number of pages9
JournalNeuroendocrinology
Volume55
Issue number4
DOIs
StatePublished - Jan 1 1992

Keywords

  • Amygdaloid body
  • Glucocorticoids
  • Hypothalamus
  • Immunocytochemistry
  • Innervation
  • Neuropeptides

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

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