TY - JOUR
T1 - Collagenous spherulosis of the breast. Immunohistochemical and ultrastructural studies
AU - Grignon, D. J.
AU - Ro, Jae
AU - Mackay, B. N.
AU - Ordonez, N. G.
AU - Ayala, Alberto
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Collagenous spherulosis of the breast is a recently described benign breast lesion that has previously been seen only incidentally. The authors report two cases of this lesion studied by immunohistochemistry and electron microscopy and a third case studied by light microscopy alone. The results demonstrate the participation of two cell types in the process: myoepithelial cells, which stained positively for cytokeratin, S-100 protein, and muscle-specific actin and contained intermediate filaments with dense bodies, pinocytotic vesicles, and formed cell junctions; and epithelial cells, which reacted for cytokeratin but not for S-100 protein or muscle-specific actin and contained intracytoplasmic lumina, formed lumina with microvilli, and desmosomes. Material constituting the spherules stained intensely for type IV collagen, and its ultrastructure was similar to that of basement-membrane material. The authors conclude that collagenous spherulosis results from a proliferation of epithelial and myoepithelial cells, the latter of which produce basement-membrane material.
AB - Collagenous spherulosis of the breast is a recently described benign breast lesion that has previously been seen only incidentally. The authors report two cases of this lesion studied by immunohistochemistry and electron microscopy and a third case studied by light microscopy alone. The results demonstrate the participation of two cell types in the process: myoepithelial cells, which stained positively for cytokeratin, S-100 protein, and muscle-specific actin and contained intermediate filaments with dense bodies, pinocytotic vesicles, and formed cell junctions; and epithelial cells, which reacted for cytokeratin but not for S-100 protein or muscle-specific actin and contained intracytoplasmic lumina, formed lumina with microvilli, and desmosomes. Material constituting the spherules stained intensely for type IV collagen, and its ultrastructure was similar to that of basement-membrane material. The authors conclude that collagenous spherulosis results from a proliferation of epithelial and myoepithelial cells, the latter of which produce basement-membrane material.
UR - http://www.scopus.com/inward/record.url?scp=0024517750&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024517750&partnerID=8YFLogxK
U2 - 10.1093/ajcp/91.4.386
DO - 10.1093/ajcp/91.4.386
M3 - Article
C2 - 2467549
AN - SCOPUS:0024517750
VL - 91
SP - 386
EP - 392
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -