TY - JOUR
T1 - Cold ischemic injury, aortic allograft vasculopathy, and pro-inflammatory cytokine expression
AU - Knight, Richard J.
AU - Liu, Hui
AU - Fishman, Eric
AU - Reis, Ernane D.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/8
Y1 - 2003/8
N2 - Background. The aim of this study was to understand the role of ischemic preservation injury and pro-inflammatory cytokine expression in the progression of allograft vasculopathy. Methods. Using the rat aortic transplant model, grafts were stored at 4°C for either 1 or 24 h. Graft vasculopathy was assessed at 4 and 8 weeks after transplantation. Intra-graft cytokine expression was measured at days 1, 3 and, 7 after transplantation. Results. At 4 weeks, intimal hyperplasia of allografts was greater than isografts (P < 0.05). At 8 weeks, all groups had an increase in graft vascular disease compared to the 4-week groups (P < 0.05). Allografts preserved for 24 h displayed a greater degree of vessel-wall reaction than both isograft groups and allografts stored for 1 h (P < 0.05). An increased expression of the cytokines, TNF-α, TGF-β, IL-2, INF-γ, IL-1, and IL-6 was noted in the allografts stored for 24 h compared to similarly treated isografts (P < 0.05). Conclusions. Prolonged ischemic preservation injury induced vascular disease in both isografts and allografts. The vessel wall reaction increased over time and was greater in allografts than isografts. The enhanced expression of T cell- and macrophage associated cytokines in allografts compared to isografts, suggested that early pro-inflammatory cytokine expression played an important role in progression of allograft vasculopathy.
AB - Background. The aim of this study was to understand the role of ischemic preservation injury and pro-inflammatory cytokine expression in the progression of allograft vasculopathy. Methods. Using the rat aortic transplant model, grafts were stored at 4°C for either 1 or 24 h. Graft vasculopathy was assessed at 4 and 8 weeks after transplantation. Intra-graft cytokine expression was measured at days 1, 3 and, 7 after transplantation. Results. At 4 weeks, intimal hyperplasia of allografts was greater than isografts (P < 0.05). At 8 weeks, all groups had an increase in graft vascular disease compared to the 4-week groups (P < 0.05). Allografts preserved for 24 h displayed a greater degree of vessel-wall reaction than both isograft groups and allografts stored for 1 h (P < 0.05). An increased expression of the cytokines, TNF-α, TGF-β, IL-2, INF-γ, IL-1, and IL-6 was noted in the allografts stored for 24 h compared to similarly treated isografts (P < 0.05). Conclusions. Prolonged ischemic preservation injury induced vascular disease in both isografts and allografts. The vessel wall reaction increased over time and was greater in allografts than isografts. The enhanced expression of T cell- and macrophage associated cytokines in allografts compared to isografts, suggested that early pro-inflammatory cytokine expression played an important role in progression of allograft vasculopathy.
KW - Allograft vasculopathy
KW - Cytokines
KW - Ischemic injury
UR - http://www.scopus.com/inward/record.url?scp=0042384382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042384382&partnerID=8YFLogxK
U2 - 10.1016/S0022-4804(03)00199-9
DO - 10.1016/S0022-4804(03)00199-9
M3 - Article
C2 - 12957130
AN - SCOPUS:0042384382
VL - 113
SP - 201
EP - 207
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 2
ER -