Coinjection strategy for visual identification of transgenic mice

Paul A. Overbeek, Estuardo Aguilar-Cordova, Gerri Hanten, David L. Schaffner, Parul Patel, Russell M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Transgenic mice were generated by coinjection of a dominant marker gene that induces fur and eye pigmentation (a tyrosinase minigene) plus an unrelated DNA construction that has a γ-glutamyl transferase (γGT) promoter linked to a ras oncogene. Mice transgenic for γGT-ras could be identified in the first and all subsequent generations by simple visual inspection for pigmentation. Furthermore, the γ-glutamyl transferase promoter was active in kidney but not skin of the transgenic mice, indicating that the cointegrated DNA was active and independently expressed. These results confirm that the tyrosinase minigene can be used for coinjections to allow rapid visual identification of transgenic mice.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalTransgenic Research
Volume1
Issue number1
DOIs
StatePublished - Dec 1991

Keywords

  • γ-glutamyl transferase
  • cointegration
  • ras T24 oncogene
  • tyrosinase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics
  • Biotechnology
  • Plant Science
  • Food Science

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