Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene

Takashi Kobayashi; Isao Inoue; Nancy A. Jenkins; Debra J. Gilbert; Neal G. Copeland; Takeshi Watanabe

doi:10.1006/geno.1996.0575

Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene

Takashi Kobayashi, Isao Inoue, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, Takeshi Watanabe

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.

Original language	English (US)
Pages (from-to)	390-394
Number of pages	5
Journal	Genomics
Volume	37
Issue number	3
DOIs	https://doi.org/10.1006/geno.1996.0575
State	Published - Nov 1 1996

ASJC Scopus subject areas

Genetics

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title = "Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene",

abstract = "A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95\% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.",

author = "Takashi Kobayashi and Isao Inoue and Jenkins, \{Nancy A.\} and Gilbert, \{Debra J.\} and Copeland, \{Neal G.\} and Takeshi Watanabe",

note = "Funding Information: We thank Drs. Masahiro Sato and Norihiro Tada for their critical reading of the manuscript and technical advice on RT-PCR and Deborah A. Barnhart and Mary Barnstead for excellent technical assistance in chromosomal mapping. This research was supported, in part, by the National Cancer Institute, DHHS, under contract with ABL. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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AU - Gilbert, Debra J.

AU - Copeland, Neal G.

AU - Watanabe, Takeshi

N1 - Funding Information: We thank Drs. Masahiro Sato and Norihiro Tada for their critical reading of the manuscript and technical advice on RT-PCR and Deborah A. Barnhart and Mary Barnstead for excellent technical assistance in chromosomal mapping. This research was supported, in part, by the National Cancer Institute, DHHS, under contract with ABL. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

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Y1 - 1996/11/1

N2 - A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.

AB - A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.

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Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene

Abstract

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