Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene

Takashi Kobayashi, Isao Inoue, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, Takeshi Watanabe

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.

Original languageEnglish (US)
Pages (from-to)390-394
Number of pages5
JournalGenomics
Volume37
Issue number3
DOIs
StatePublished - Nov 1 1996

ASJC Scopus subject areas

  • Genetics

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