Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Linda S. Park; Unja Martin; Kirsten Garka; Brian Gliniak; James P. Di Santo; Werner Muller; David A. Largaespada; Neal G. Copeland; Nancy A. Jenkins; Andrew G. Farr; Steven F. Ziegler; Philip J. Morrissey; Raymond Paxton; John E. Sims

doi:10.1084/jem.192.5.659

Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Linda S. Park, Unja Martin, Kirsten Garka, Brian Gliniak, James P. Di Santo, Werner Muller, David A. Largaespada, Neal G. Copeland, Nancy A. Jenkins, Andrew G. Farr, Steven F. Ziegler, Philip J. Morrissey, Raymond Paxton, John E. Sims

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348 Scopus citations

Abstract

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.

Original language	English (US)
Pages (from-to)	659-669
Number of pages	11
Journal	Journal of Experimental Medicine
Volume	192
Issue number	5
DOIs	https://doi.org/10.1084/jem.192.5.659
State	Published - Sep 4 2000

Keywords

Cytokine
Cytokine receptors
DNA sequence
Interleukin 2 receptor
Interleukin 7 receptor

ASJC Scopus subject areas

Immunology

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10.1084/jem.192.5.659

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Park, L. S., Martin, U., Garka, K., Gliniak, B., Di Santo, J. P., Muller, W., Largaespada, D. A., Copeland, N. G., Jenkins, N. A., Farr, A. G., Ziegler, S. F., Morrissey, P. J., Paxton, R., & Sims, J. E. (2000). Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. Journal of Experimental Medicine, 192(5), 659-669. https://doi.org/10.1084/jem.192.5.659

Park, LS, Martin, U, Garka, K, Gliniak, B, Di Santo, JP, Muller, W, Largaespada, DA, Copeland, NG , Jenkins, NA, Farr, AG, Ziegler, SF, Morrissey, PJ, Paxton, R & Sims, JE 2000, 'Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor', Journal of Experimental Medicine, vol. 192, no. 5, pp. 659-669. https://doi.org/10.1084/jem.192.5.659

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title = "Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor",

abstract = "The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.",

keywords = "Cytokine, Cytokine receptors, DNA sequence, Interleukin 2 receptor, Interleukin 7 receptor",

author = "Park, {Linda S.} and Unja Martin and Kirsten Garka and Brian Gliniak and {Di Santo}, {James P.} and Werner Muller and Largaespada, {David A.} and Copeland, {Neal G.} and Jenkins, {Nancy A.} and Farr, {Andrew G.} and Ziegler, {Steven F.} and Morrissey, {Philip J.} and Raymond Paxton and Sims, {John E.}",

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AU - Jenkins, Nancy A.

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AU - Ziegler, Steven F.

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Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

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