Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Linda S. Park, Unja Martin, Kirsten Garka, Brian Gliniak, James P. Di Santo, Werner Muller, David A. Largaespada, Neal G. Copeland, Nancy A. Jenkins, Andrew G. Farr, Steven F. Ziegler, Philip J. Morrissey, Raymond Paxton, John E. Sims

Research output: Contribution to journalArticle

303 Scopus citations

Abstract

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.

Original languageEnglish (US)
Pages (from-to)659-669
Number of pages11
JournalJournal of Experimental Medicine
Volume192
Issue number5
DOIs
StatePublished - Sep 4 2000

Keywords

  • Cytokine
  • Cytokine receptors
  • DNA sequence
  • Interleukin 2 receptor
  • Interleukin 7 receptor

ASJC Scopus subject areas

  • Immunology

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