TY - JOUR
T1 - Cloning of the cDNA encoding rod photoreceptor cGMP-phosphodiesterase α and γ subunits from the retinal degenerate Labrador Retriever dog
AU - Kylmä, T.
AU - Paulin, L.
AU - Hurwitz, M. Y.
AU - Hurwitz, Richard
AU - Kommonen, B.
N1 - Funding Information:
This work has been supported by grants from the Finnish Government to TK and BK and NEI Grant EY06656 to RLH. We thank P~iivi Laamanen for excellent technical assistance in DNA sequencing.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997
Y1 - 1997
N2 - The nucleotide (nt) sequence of the cDNA encoding the retinal rod cyclic 3′5′-GMP phosphodiesterase (PDE) α and γ subunits from two strains of dogs - (i) Labrador Retrievers homozygous for autosomally recessively inherited rod-cone degeneration and (ii) the wild-type Beagle - are reported. Cloning of these subunits was accomplished by polymerase chain reaction using retinal cDNA libraries as templates. The nt sequence of α PDE predicts a 861-amino-acid polypeptide which is 97-7 per cent and 96-9 per cent identical to the bovine and human counterparts, respectively. PDE γ encodes an 87-amino-acid polypeptide differing from bovine and murine gamma subunits by only one amino acid. Since no differences were found between these two strains of dogs, the cause of the Labrador Retriever's degeneration remains to be determined.
AB - The nucleotide (nt) sequence of the cDNA encoding the retinal rod cyclic 3′5′-GMP phosphodiesterase (PDE) α and γ subunits from two strains of dogs - (i) Labrador Retrievers homozygous for autosomally recessively inherited rod-cone degeneration and (ii) the wild-type Beagle - are reported. Cloning of these subunits was accomplished by polymerase chain reaction using retinal cDNA libraries as templates. The nt sequence of α PDE predicts a 861-amino-acid polypeptide which is 97-7 per cent and 96-9 per cent identical to the bovine and human counterparts, respectively. PDE γ encodes an 87-amino-acid polypeptide differing from bovine and murine gamma subunits by only one amino acid. Since no differences were found between these two strains of dogs, the cause of the Labrador Retriever's degeneration remains to be determined.
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U2 - 10.1016/S0034-5288(97)90208-3
DO - 10.1016/S0034-5288(97)90208-3
M3 - Article
C2 - 9300552
AN - SCOPUS:0031132442
VL - 62
SP - 293
EP - 296
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 3
ER -