Cloning of the cDNA encoding rod photoreceptor cGMP-phosphodiesterase α and γ subunits from the retinal degenerate Labrador Retriever dog

T. Kylmä, L. Paulin, M. Y. Hurwitz, Richard Hurwitz, B. Kommonen

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The nucleotide (nt) sequence of the cDNA encoding the retinal rod cyclic 3′5′-GMP phosphodiesterase (PDE) α and γ subunits from two strains of dogs - (i) Labrador Retrievers homozygous for autosomally recessively inherited rod-cone degeneration and (ii) the wild-type Beagle - are reported. Cloning of these subunits was accomplished by polymerase chain reaction using retinal cDNA libraries as templates. The nt sequence of α PDE predicts a 861-amino-acid polypeptide which is 97-7 per cent and 96-9 per cent identical to the bovine and human counterparts, respectively. PDE γ encodes an 87-amino-acid polypeptide differing from bovine and murine gamma subunits by only one amino acid. Since no differences were found between these two strains of dogs, the cause of the Labrador Retriever's degeneration remains to be determined.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalResearch in Veterinary Science
Volume62
Issue number3
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • veterinary(all)

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