Cloning of cDNA and genomic structure of the mouse γ-glutamyl transpeptidase-encoding gene

Zheng-Zheng Shi, Geetha M. Habib, Russell M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have isolated and characterized cDNA and genomic clones containing the coding region for the mouse γ-glutamyl transpeptidase (GGT). The sequences of the full-length cDNAs for three of the seven known mouse Ggt RNAs (types I, II and III) were determined and found to be identical in the coding region. Comparisons of the deduced amino-acid sequence of mouse GGT with that of rat and human reveal 95 and 79% overall identities, respectively. The mouse Ggt gene has 12 coding exons and spans approx. 12 kb. We have also re-analyzed rat genomic Ggt clones previously isolated by us and found that the rat and mouse genes share the same intron/exon boundaries. Our findings are of interest because they define the structure of the mouse and rat Ggt genes and will allow comparison with human GGT genes which, recent findings suggest, have diverged substantially from rodents.

Original languageEnglish (US)
Pages (from-to)233-237
Number of pages5
JournalGene
Volume167
Issue number1-2
DOIs
StatePublished - Dec 29 1995

Keywords

  • amino-acid sequence
  • rat
  • Recombinant DNA
  • splice junction

ASJC Scopus subject areas

  • Genetics

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