Cloning of cDNA and genomic structure of the mouse γ-glutamyl transpeptidase-encoding gene

Zheng-Zheng Shi; Geetha M. Habib; Russell M. Lebovitz; Michael W. Lieberman

doi:10.1016/0378-1119(95)00618-4

Cloning of cDNA and genomic structure of the mouse γ-glutamyl transpeptidase-encoding gene

Zheng-Zheng Shi, Geetha M. Habib, Russell M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We have isolated and characterized cDNA and genomic clones containing the coding region for the mouse γ-glutamyl transpeptidase (GGT). The sequences of the full-length cDNAs for three of the seven known mouse Ggt RNAs (types I, II and III) were determined and found to be identical in the coding region. Comparisons of the deduced amino-acid sequence of mouse GGT with that of rat and human reveal 95 and 79% overall identities, respectively. The mouse Ggt gene has 12 coding exons and spans approx. 12 kb. We have also re-analyzed rat genomic Ggt clones previously isolated by us and found that the rat and mouse genes share the same intron/exon boundaries. Our findings are of interest because they define the structure of the mouse and rat Ggt genes and will allow comparison with human GGT genes which, recent findings suggest, have diverged substantially from rodents.

Original language	English (US)
Pages (from-to)	233-237
Number of pages	5
Journal	Gene
Volume	167
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(95)00618-4
State	Published - Dec 29 1995

Keywords

amino-acid sequence
rat
Recombinant DNA
splice junction

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(95)00618-4

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title = "Cloning of cDNA and genomic structure of the mouse γ-glutamyl transpeptidase-encoding gene",

abstract = "We have isolated and characterized cDNA and genomic clones containing the coding region for the mouse γ-glutamyl transpeptidase (GGT). The sequences of the full-length cDNAs for three of the seven known mouse Ggt RNAs (types I, II and III) were determined and found to be identical in the coding region. Comparisons of the deduced amino-acid sequence of mouse GGT with that of rat and human reveal 95 and 79% overall identities, respectively. The mouse Ggt gene has 12 coding exons and spans approx. 12 kb. We have also re-analyzed rat genomic Ggt clones previously isolated by us and found that the rat and mouse genes share the same intron/exon boundaries. Our findings are of interest because they define the structure of the mouse and rat Ggt genes and will allow comparison with human GGT genes which, recent findings suggest, have diverged substantially from rodents.",

keywords = "amino-acid sequence, rat, Recombinant DNA, splice junction",

author = "Zheng-Zheng Shi and Habib, {Geetha M.} and Lebovitz, {Russell M.} and Lieberman, {Michael W.}",

note = "Funding Information: This work was supported by NIH Grant CA-39392.",

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doi = "10.1016/0378-1119(95)00618-4",

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T1 - Cloning of cDNA and genomic structure of the mouse γ-glutamyl transpeptidase-encoding gene

AU - Shi, Zheng-Zheng

AU - Habib, Geetha M.

AU - Lebovitz, Russell M.

AU - Lieberman, Michael W.

N1 - Funding Information: This work was supported by NIH Grant CA-39392.

PY - 1995/12/29

Y1 - 1995/12/29

N2 - We have isolated and characterized cDNA and genomic clones containing the coding region for the mouse γ-glutamyl transpeptidase (GGT). The sequences of the full-length cDNAs for three of the seven known mouse Ggt RNAs (types I, II and III) were determined and found to be identical in the coding region. Comparisons of the deduced amino-acid sequence of mouse GGT with that of rat and human reveal 95 and 79% overall identities, respectively. The mouse Ggt gene has 12 coding exons and spans approx. 12 kb. We have also re-analyzed rat genomic Ggt clones previously isolated by us and found that the rat and mouse genes share the same intron/exon boundaries. Our findings are of interest because they define the structure of the mouse and rat Ggt genes and will allow comparison with human GGT genes which, recent findings suggest, have diverged substantially from rodents.

AB - We have isolated and characterized cDNA and genomic clones containing the coding region for the mouse γ-glutamyl transpeptidase (GGT). The sequences of the full-length cDNAs for three of the seven known mouse Ggt RNAs (types I, II and III) were determined and found to be identical in the coding region. Comparisons of the deduced amino-acid sequence of mouse GGT with that of rat and human reveal 95 and 79% overall identities, respectively. The mouse Ggt gene has 12 coding exons and spans approx. 12 kb. We have also re-analyzed rat genomic Ggt clones previously isolated by us and found that the rat and mouse genes share the same intron/exon boundaries. Our findings are of interest because they define the structure of the mouse and rat Ggt genes and will allow comparison with human GGT genes which, recent findings suggest, have diverged substantially from rodents.

KW - amino-acid sequence

KW - rat

KW - Recombinant DNA

KW - splice junction

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JO - Gene

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ER -

Cloning of cDNA and genomic structure of the mouse γ-glutamyl transpeptidase-encoding gene

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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