Cloning. Chromosomal localization and expression pattern of the POU domain gene Oct-11

Andrew S. Goldsborough; Lyn E. Healy; Neal G. Copeland; Debra J. Gilbert; Nancy A. Jenkins; Keith R. Willison; Alan Ashworth

doi:10.1093/nar/21.1.127

Cloning. Chromosomal localization and expression pattern of the POU domain gene Oct-11

Andrew S. Goldsborough, Lyn E. Healy, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Keith R. Willison, Alan Ashworth

Research output: Contribution to journal › Article

38 Scopus citations

Abstract

POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes. We have cloned and sequenced cDNAs encoding a novel mouse POU domain protein, Oct-11, that is closely related within the POU domain to the POU class II proteins, Oct-1 and Oct-2. Recombinant Oc t-11 protein binds specifically to an octamer sequence in vitro. The Oct-11 gene is expressed during mouse embryogenesis and in the adult thymus and testis. In addition, it is abundant in the myeloma cell line P3/NS-1/1-Ag4.1. We describe the structure of Oct-11 and its chromosomal localization, and discuss the evidence that the POU class II gene family has evolved by duplication and divergence of a common ancestral gene.

Original language	English (US)
Pages (from-to)	127-134
Number of pages	8
Journal	Nucleic Acids Research
Volume	21
Issue number	1
DOIs	https://doi.org/10.1093/nar/21.1.127
State	Published - Jan 11 1993

ASJC Scopus subject areas

Genetics

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title = "Cloning. Chromosomal localization and expression pattern of the POU domain gene Oct-11",

abstract = "POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes. We have cloned and sequenced cDNAs encoding a novel mouse POU domain protein, Oct-11, that is closely related within the POU domain to the POU class II proteins, Oct-1 and Oct-2. Recombinant Oc t-11 protein binds specifically to an octamer sequence in vitro. The Oct-11 gene is expressed during mouse embryogenesis and in the adult thymus and testis. In addition, it is abundant in the myeloma cell line P3/NS-1/1-Ag4.1. We describe the structure of Oct-11 and its chromosomal localization, and discuss the evidence that the POU class II gene family has evolved by duplication and divergence of a common ancestral gene.",

author = "Goldsborough, {Andrew S.} and Healy, {Lyn E.} and Copeland, {Neal G.} and Gilbert, {Debra J.} and Jenkins, {Nancy A.} and Willison, {Keith R.} and Alan Ashworth",

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AU - Goldsborough, Andrew S.

AU - Healy, Lyn E.

AU - Copeland, Neal G.

AU - Gilbert, Debra J.

AU - Jenkins, Nancy A.

AU - Willison, Keith R.

AU - Ashworth, Alan

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AB - POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes. We have cloned and sequenced cDNAs encoding a novel mouse POU domain protein, Oct-11, that is closely related within the POU domain to the POU class II proteins, Oct-1 and Oct-2. Recombinant Oc t-11 protein binds specifically to an octamer sequence in vitro. The Oct-11 gene is expressed during mouse embryogenesis and in the adult thymus and testis. In addition, it is abundant in the myeloma cell line P3/NS-1/1-Ag4.1. We describe the structure of Oct-11 and its chromosomal localization, and discuss the evidence that the POU class II gene family has evolved by duplication and divergence of a common ancestral gene.

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