Cloning and Structure of the Human Corticotrophin Releasing Factor-Binding Protein Gene (CRHBP)

Dominic P. Behan, Ellen Potter, Kathy A. Lewis, Nancy A. Jenkins, Neal Copeland, Philip J. Lowry, Wylie W. Vale

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

The human CRF-binding protein gene has been cloned and mapped to the distal region of chromosome 13 and loci 5q in the mouse and human genomes, respectively. The gene consists of 7 exons and 6 introns. The mature protein has 10 cysteines and 5 tandem disulfide bridges 4 of which are contained within exons 3, 5, 6, and 7. One bridge is shared by exons 3 and 4. The signal peptide and the first 3 amino acids of the mature protein were coded for by an extreme 5' exon. Primer extension analyses revealed the transcriptional initiation site to be located 32 bp downstream from a consensus TATA box. The promoter sequence contained a number of putative promoter elements including an AP-l site, three ER-half sites, the immunoglobulin enhancer elements NF-κB and INF-l, and the liver-specific enhancers LFAl and LFB1.

Original languageEnglish (US)
Pages (from-to)63-68
Number of pages6
JournalGenomics
Volume16
Issue number1
DOIs
StatePublished - Apr 1993

ASJC Scopus subject areas

  • Genetics

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