TY - JOUR
T1 - Cloning and regulation by glucocorticoid receptor ligands of a rat hsp90
AU - McGuire, Jacqueline A.
AU - Poellinger, Lorenz
AU - Wikström, Ann Charlotte
AU - Gustafsson, Jan Åke
N1 - Funding Information:
Acknowledgements--We would like to thank Dr Catherine Legrave~ead and Marika R6nnhohn for methodological advice, Dr Marc Denis for initial help in this study and Smanne ~ n for excellent technical asmtance. The OR probe was a generous gift from Dr Sam Okret (Stockholm). The TAT eDNA probe was a generous gift from Profe~tor Gunther Shutz (Heidelberg). This work was supported by a grant from the Swedish Medicai Research Council (No. 13x-2819). J.A.M. is a recipient of a training fellowship from the Swedish Cancer Society.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/9
Y1 - 1992/9
N2 - We have isolated a full length cDNA that encodes a heat shock protein, hsp90, from a rat brain library and present the nucleotide sequence and deduced amino acid sequence. Comparison of the entire nucleotide sequence with mouse hsp84 and human hsp90β cDNAs reveal sequence similarities of 92 and 87%, respectively. The coding region of 2172 nucleotides corresponds to a polypeptide chain of 724 amino acids. Comparison with mouse hsp84 and human hsp90β amino acid sequences indicates a similarity of 97%, respectively. Characterization of the constitutive expression of this cDNA both by RNA blot hybridization and immunoblotting, reveals that it is expressed in all rat tissues examined. Hsp90 has been shown to form a transient complex with steroid hormone receptors. In order to further elucidate the role of hsp90 in the endocrine response of cells, we have examined the effects of dexamethasone and RU38486 on the level of hsp90 mRNA in a system in which glucocorticoids down-regulate glucocorticoid receptor mRNA levels. In this system, a subtle but reproducible approx. 2-fold decrease in hsp90 mRNA levels is observed after 48 h treatment with dexamethasone.
AB - We have isolated a full length cDNA that encodes a heat shock protein, hsp90, from a rat brain library and present the nucleotide sequence and deduced amino acid sequence. Comparison of the entire nucleotide sequence with mouse hsp84 and human hsp90β cDNAs reveal sequence similarities of 92 and 87%, respectively. The coding region of 2172 nucleotides corresponds to a polypeptide chain of 724 amino acids. Comparison with mouse hsp84 and human hsp90β amino acid sequences indicates a similarity of 97%, respectively. Characterization of the constitutive expression of this cDNA both by RNA blot hybridization and immunoblotting, reveals that it is expressed in all rat tissues examined. Hsp90 has been shown to form a transient complex with steroid hormone receptors. In order to further elucidate the role of hsp90 in the endocrine response of cells, we have examined the effects of dexamethasone and RU38486 on the level of hsp90 mRNA in a system in which glucocorticoids down-regulate glucocorticoid receptor mRNA levels. In this system, a subtle but reproducible approx. 2-fold decrease in hsp90 mRNA levels is observed after 48 h treatment with dexamethasone.
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U2 - 10.1016/0960-0760(92)90089-2
DO - 10.1016/0960-0760(92)90089-2
M3 - Article
C2 - 1525042
AN - SCOPUS:0026781502
SN - 0960-0760
VL - 42
SP - 813
EP - 822
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
IS - 8
ER -