Cloning and regulation by glucocorticoid receptor ligands of a rat hsp90

Jacqueline A. McGuire, Lorenz Poellinger, Ann Charlotte Wikström, Jan Åke Gustafsson

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have isolated a full length cDNA that encodes a heat shock protein, hsp90, from a rat brain library and present the nucleotide sequence and deduced amino acid sequence. Comparison of the entire nucleotide sequence with mouse hsp84 and human hsp90β cDNAs reveal sequence similarities of 92 and 87%, respectively. The coding region of 2172 nucleotides corresponds to a polypeptide chain of 724 amino acids. Comparison with mouse hsp84 and human hsp90β amino acid sequences indicates a similarity of 97%, respectively. Characterization of the constitutive expression of this cDNA both by RNA blot hybridization and immunoblotting, reveals that it is expressed in all rat tissues examined. Hsp90 has been shown to form a transient complex with steroid hormone receptors. In order to further elucidate the role of hsp90 in the endocrine response of cells, we have examined the effects of dexamethasone and RU38486 on the level of hsp90 mRNA in a system in which glucocorticoids down-regulate glucocorticoid receptor mRNA levels. In this system, a subtle but reproducible approx. 2-fold decrease in hsp90 mRNA levels is observed after 48 h treatment with dexamethasone.

Original languageEnglish (US)
Pages (from-to)813-822
Number of pages10
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume42
Issue number8
DOIs
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'Cloning and regulation by glucocorticoid receptor ligands of a rat hsp90'. Together they form a unique fingerprint.

Cite this