Cloning and pretranslation hormonal regulation of testosterone 16α-hydroxylase (P-450(16α)) in male rat liver

A. Strom, A. Mode, P. Zaphiropoulos, A. G. Nilsson, E. Morgan, Jan-Ake Gustafsson

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

cDNA clones for P-450(16α) were isolated from a male liver λgt11 expression library using antibodies against P-450(16α). The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-450(16α) in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-450(16α).

Original languageEnglish (US)
Pages (from-to)314-320
Number of pages7
JournalActa endocrinologica
Volume118
Issue number2
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Endocrinology

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