Cloning and pretranslation hormonal regulation of testosterone 16α-hydroxylase (P-450(16α)) in male rat liver

A. Strom; A. Mode; P. Zaphiropoulos; A. G. Nilsson; E. Morgan; J. A. Gustafsson

Cloning and pretranslation hormonal regulation of testosterone 16α-hydroxylase (P-450(16α)) in male rat liver

A. Strom, A. Mode, P. Zaphiropoulos, A. G. Nilsson, E. Morgan, J. A. Gustafsson

Research output: Contribution to journal › Article › peer-review

Abstract

cDNA clones for P-450(16α) were isolated from a male liver λgt11 expression library using antibodies against P-450(16α). The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH₂-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-450(16α) in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-450(16α).

Original language	English (US)
Pages (from-to)	314-320
Number of pages	7
Journal	Acta endocrinologica
Volume	118
Issue number	2
State	Published - 1988

ASJC Scopus subject areas

Endocrinology

Cite this

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title = "Cloning and pretranslation hormonal regulation of testosterone 16α-hydroxylase (P-450(16α)) in male rat liver",

abstract = "cDNA clones for P-450(16α) were isolated from a male liver λgt11 expression library using antibodies against P-450(16α). The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-450(16α) in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-450(16α).",

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T1 - Cloning and pretranslation hormonal regulation of testosterone 16α-hydroxylase (P-450(16α)) in male rat liver

AU - Strom, A.

AU - Mode, A.

AU - Zaphiropoulos, P.

AU - Nilsson, A. G.

AU - Morgan, E.

AU - Gustafsson, J. A.

PY - 1988

Y1 - 1988

N2 - cDNA clones for P-450(16α) were isolated from a male liver λgt11 expression library using antibodies against P-450(16α). The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-450(16α) in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-450(16α).

AB - cDNA clones for P-450(16α) were isolated from a male liver λgt11 expression library using antibodies against P-450(16α). The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-450(16α) in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-450(16α).

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Cloning and pretranslation hormonal regulation of testosterone 16α-hydroxylase (P-450(16α)) in male rat liver

Abstract

ASJC Scopus subject areas

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