cDNA clones for P-450(16α) were isolated from a male liver λgt11 expression library using antibodies against P-450(16α). The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-450(16α) in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-450(16α).
|Original language||English (US)|
|Number of pages||7|
|State||Published - Jan 1 1988|
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