Cloning and expression analysis of murine phospholipase D1

William C. Colley, Yelena M. Altshuller, Christopher K. Sue-Ling, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Kimberly D. Branch, Styliani E. Tsirka, Roni J. Bollag, Wendy B. Bollag, Michael A. Frohman

Research output: Contribution to journalArticlepeer-review

115 Scopus citations


Activation of phosphatidylcholine-specific phospholipase D (PLD) occurs as part of the complex signal-transduction cascade initiated by agonist stimulation of tyrosine kinase and G-protein-coupled receptors. A variety of mammalian PLD activities have been described, and cDNAs for two PLDs recently reported (human PLD1 and murine PLD2). We describe here the cloning and chromosomal localization of murine PLD1. Northern-blot hybridization and RNase protection analyses were used to examine the expression of murine PLD1 and PLD2 in a variety of cell lines and tissues. PLD1 and PLD2 were expressed in all RNA samples examined, although the absolute expression of each isoform varied, as well as the ratio of PLD1 to PLD2. Moreover, in situ hybridization of adult brain and murine embryo sections revealed high levels of expression of individual PLDs in some cell types and no detectable expression in others. Thus the two PLDs probably carry out distinct roles in restricted subsets of cells rather than ubiquitous roles in all cells.

Original languageEnglish (US)
Pages (from-to)745-753
Number of pages9
JournalBiochemical Journal
Issue number3
StatePublished - Sep 15 1997

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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