TY - JOUR
T1 - Cloning and characterization of the mouse alpha globin cluster and a new hypervariable marker
AU - Zhao, Q. Z.
AU - Liang, X. L.
AU - Mitra, Sankar
AU - Gourdon, G.
AU - Alter, B. P.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - A 95-kb region of the mouse genome spanning the entire α-globin gene cluster was isolated as overlapping cosmid clones and characterized. In addition to the embryonic (ζ) and adult (α) genes, the cloned contig contains the complete N-methylpurine-DNA glycosylase (MPG) gene, the α-globin-positive regulatory element (mHS-26), and a previously unidentified hypervariable region (named the mouse α-HVR). In mice, the distance between the MPG gene and mHS-26 is ∼18 kb; between the mHS-26 and the ζ-gene, ∼26 kb; from the ζ-gene to the 5′ end of the α-gene, ∼16 kb; and the two α-genes are separated by ∼12 kb. In human, the corresponding distances are ∼27 kb, ∼40 kb, ∼19 kb, and ∼3 kb respectively. The α-HVR is located ∼18 kb upstream of the mouse ζ-globin gene transcription start site and contains a variable copy number tandem repeat (VNTR) array of a 35-bp sequence rich in (G+C) content. The unit sequence of the HVR shares the short core sequence with the HVRs identified in the human α-gene cluster. Thus, this HVR may be a valuable evolutionary marker, as well as a useful genetic marker for the mouse.
AB - A 95-kb region of the mouse genome spanning the entire α-globin gene cluster was isolated as overlapping cosmid clones and characterized. In addition to the embryonic (ζ) and adult (α) genes, the cloned contig contains the complete N-methylpurine-DNA glycosylase (MPG) gene, the α-globin-positive regulatory element (mHS-26), and a previously unidentified hypervariable region (named the mouse α-HVR). In mice, the distance between the MPG gene and mHS-26 is ∼18 kb; between the mHS-26 and the ζ-gene, ∼26 kb; from the ζ-gene to the 5′ end of the α-gene, ∼16 kb; and the two α-genes are separated by ∼12 kb. In human, the corresponding distances are ∼27 kb, ∼40 kb, ∼19 kb, and ∼3 kb respectively. The α-HVR is located ∼18 kb upstream of the mouse ζ-globin gene transcription start site and contains a variable copy number tandem repeat (VNTR) array of a 35-bp sequence rich in (G+C) content. The unit sequence of the HVR shares the short core sequence with the HVRs identified in the human α-gene cluster. Thus, this HVR may be a valuable evolutionary marker, as well as a useful genetic marker for the mouse.
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U2 - 10.1007/s003359900225
DO - 10.1007/s003359900225
M3 - Article
C2 - 8854862
AN - SCOPUS:0030253167
SN - 0938-8990
VL - 7
SP - 749
EP - 753
JO - Mammalian Genome
JF - Mammalian Genome
IS - 10
ER -