TY - JOUR
T1 - Cloning and characterization of RAP250, a novel nuclear receptor coactivator
AU - Caira, Françoise
AU - Antonson, Per
AU - Pelto-Huikko, Markku
AU - Treuter, Eckardt
AU - Gustafsson, Jan Åke
PY - 2000/2/25
Y1 - 2000/2/25
N2 - Ligand-induced transcriptional activation of gene expression by nuclear receptors is dependent on recruitment of coactivators as intermediary factors. The present work describes the cloning and characterization of RAP250, a novel human nuclear receptor coactivator. The results of in vitro and in vivo experiments indicate that the interaction of RAP250 with nuclear receptors is ligand-dependent or ligand-enhanced depending on the nuclear receptor and involves only one short LXXLL motif called nuclear receptor box. Transient transfection assays further demonstrate that RAP250 has a large intrinsic glutamine-rich activation domain and can significantly enhance the transcriptional activity of several nuclear receptors, acting as a coactivator. Interestingly, Northern blot and in situ hybridization analyses reveal that RAP250 is widely expressed with the highest expression in reproductive organs (testis, prostate and ovary) and brain. Together, our data suggest that RAP250 may play an important role in mammalian gene expression mediated by nuclear receptor.
AB - Ligand-induced transcriptional activation of gene expression by nuclear receptors is dependent on recruitment of coactivators as intermediary factors. The present work describes the cloning and characterization of RAP250, a novel human nuclear receptor coactivator. The results of in vitro and in vivo experiments indicate that the interaction of RAP250 with nuclear receptors is ligand-dependent or ligand-enhanced depending on the nuclear receptor and involves only one short LXXLL motif called nuclear receptor box. Transient transfection assays further demonstrate that RAP250 has a large intrinsic glutamine-rich activation domain and can significantly enhance the transcriptional activity of several nuclear receptors, acting as a coactivator. Interestingly, Northern blot and in situ hybridization analyses reveal that RAP250 is widely expressed with the highest expression in reproductive organs (testis, prostate and ovary) and brain. Together, our data suggest that RAP250 may play an important role in mammalian gene expression mediated by nuclear receptor.
UR - http://www.scopus.com/inward/record.url?scp=0034007998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034007998&partnerID=8YFLogxK
U2 - 10.1074/jbc.275.8.5308
DO - 10.1074/jbc.275.8.5308
M3 - Article
C2 - 10681503
AN - SCOPUS:0034007998
VL - 275
SP - 5308
EP - 5317
JO - The Journal of biological chemistry
JF - The Journal of biological chemistry
SN - 0021-9258
IS - 8
ER -