Cloning and analysis of the rat γ-glutamyltransferase gene

S. Rajagopalan, J. H. Park, P. D. Patel, R. M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We have isolated and characterized a complete structural gene encoding the enzyme γ-glutamyltransferase ((5-glutamyl)-peptide:amino acid 5-glutamyltransferase; EC 2.3.2.2). The gene contains 8 exons and spans ∼12 kilobases. Ras-transformed rat liver epithelial cells and rat kidney express RNAs which differ in length by ∼0.3 kilobase pair. Comparison of the genomic sequence with kidney γGT cDNA sequence indicates that the first exon is noncoding, and nuclease protection and primer extension data have identified a potential kidney transcription start site (defined as +1) for this exon. The site is not associated with a TATA box, but there are two CCAAT boxes (-136 and -599) and two sites (-101 and -746) containing the consensus sequences to which the transcription factor SP1 is known to bind. There is also a sequence at -453 (TGTGGTTG) that is highly homologous to the core sequence (TGTGG(T)3-5G) of SV40 and polyoma viral enhancers.

Original languageEnglish (US)
Pages (from-to)11721-11725
Number of pages5
JournalJournal of Biological Chemistry
Volume265
Issue number20
StatePublished - 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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