Cloning and analysis of the cDNA encoding the rod G-protein transducin α, β1 and γ1 subunits from the canine retina

Tarja Kylmä, Lars Paulin, Mary Y. Hurwitz, Richard Hurwitz, Bertel Kommonen

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The canine (Canis familiaris) retinal rod transducin (G(T)) α, β1 and γ1 subunits were sequenced. Cloning of the cDNAs was accomplished by polymerase chain reaction (PCR) using degenerate and wild type retinal cDNA libraries as templates. The deduced amino acid sequences were highly similar to rod transducins from other species: G(Tα) differed by 5 amino acids from the corresponding human sequence, whereas β1 and γ1 were identical to human sequences. The coding sequence of rod transducin was evaluated as a possible cause for the recessively inherited retinal rod-cone degeneration: there were no nucleotide differences between the wild type and retinal degenerate strains.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalGene
Volume193
Issue number1
DOIs
StatePublished - Aug 2 1997

Keywords

  • Canis familiaris
  • Dog model
  • Phototransduction

ASJC Scopus subject areas

  • Genetics

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