Cloning and analysis of the cDNA encoding the rod G-protein transducin α, β1 and γ1 subunits from the canine retina

Tarja Kylmä; Lars Paulin; Mary Y. Hurwitz; Richard Hurwitz; Bertel Kommonen

doi:10.1016/S0378-1119(97)00060-7

Cloning and analysis of the cDNA encoding the rod G-protein transducin α, β1 and γ1 subunits from the canine retina

Tarja Kylmä, Lars Paulin, Mary Y. Hurwitz, Richard Hurwitz, Bertel Kommonen

Houston Methodist

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The canine (Canis familiaris) retinal rod transducin (G(T)) α, β1 and γ1 subunits were sequenced. Cloning of the cDNAs was accomplished by polymerase chain reaction (PCR) using degenerate and wild type retinal cDNA libraries as templates. The deduced amino acid sequences were highly similar to rod transducins from other species: G(Tα) differed by 5 amino acids from the corresponding human sequence, whereas β1 and γ1 were identical to human sequences. The coding sequence of rod transducin was evaluated as a possible cause for the recessively inherited retinal rod-cone degeneration: there were no nucleotide differences between the wild type and retinal degenerate strains.

Original language	English (US)
Pages (from-to)	1-4
Number of pages	4
Journal	Gene
Volume	193
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1119(97)00060-7
State	Published - 1997

Keywords

Canis familiaris
Dog model
Phototransduction

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/S0378-1119(97)00060-7

Cite this

@article{cea43721676c4d2cae8daf28734d9e56,

title = "Cloning and analysis of the cDNA encoding the rod G-protein transducin α, β1 and γ1 subunits from the canine retina",

abstract = "The canine (Canis familiaris) retinal rod transducin (G(T)) α, β1 and γ1 subunits were sequenced. Cloning of the cDNAs was accomplished by polymerase chain reaction (PCR) using degenerate and wild type retinal cDNA libraries as templates. The deduced amino acid sequences were highly similar to rod transducins from other species: G(Tα) differed by 5 amino acids from the corresponding human sequence, whereas β1 and γ1 were identical to human sequences. The coding sequence of rod transducin was evaluated as a possible cause for the recessively inherited retinal rod-cone degeneration: there were no nucleotide differences between the wild type and retinal degenerate strains.",

keywords = "Canis familiaris, Dog model, Phototransduction",

author = "Tarja Kylm{\"a} and Lars Paulin and Hurwitz, {Mary Y.} and Richard Hurwitz and Bertel Kommonen",

note = "Funding Information: We thank Dr. Ted Wensel for preliminary primer 8820 and P{\"a}ivi Laamanen for excellent technical assistance in DNA sequencing. This work has been supported by grants from the Finnish Government to T.K. and B.K., and NEI Grant EY06656 to R.L.H. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1997",

doi = "10.1016/S0378-1119(97)00060-7",

language = "English (US)",

volume = "193",

pages = "1--4",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Cloning and analysis of the cDNA encoding the rod G-protein transducin α, β1 and γ1 subunits from the canine retina

AU - Kylmä, Tarja

AU - Paulin, Lars

AU - Hurwitz, Mary Y.

AU - Hurwitz, Richard

AU - Kommonen, Bertel

N1 - Funding Information: We thank Dr. Ted Wensel for preliminary primer 8820 and Päivi Laamanen for excellent technical assistance in DNA sequencing. This work has been supported by grants from the Finnish Government to T.K. and B.K., and NEI Grant EY06656 to R.L.H. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1997

Y1 - 1997

N2 - The canine (Canis familiaris) retinal rod transducin (G(T)) α, β1 and γ1 subunits were sequenced. Cloning of the cDNAs was accomplished by polymerase chain reaction (PCR) using degenerate and wild type retinal cDNA libraries as templates. The deduced amino acid sequences were highly similar to rod transducins from other species: G(Tα) differed by 5 amino acids from the corresponding human sequence, whereas β1 and γ1 were identical to human sequences. The coding sequence of rod transducin was evaluated as a possible cause for the recessively inherited retinal rod-cone degeneration: there were no nucleotide differences between the wild type and retinal degenerate strains.

AB - The canine (Canis familiaris) retinal rod transducin (G(T)) α, β1 and γ1 subunits were sequenced. Cloning of the cDNAs was accomplished by polymerase chain reaction (PCR) using degenerate and wild type retinal cDNA libraries as templates. The deduced amino acid sequences were highly similar to rod transducins from other species: G(Tα) differed by 5 amino acids from the corresponding human sequence, whereas β1 and γ1 were identical to human sequences. The coding sequence of rod transducin was evaluated as a possible cause for the recessively inherited retinal rod-cone degeneration: there were no nucleotide differences between the wild type and retinal degenerate strains.

KW - Canis familiaris

KW - Dog model

KW - Phototransduction

UR - http://www.scopus.com/inward/record.url?scp=0030744154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030744154&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(97)00060-7

DO - 10.1016/S0378-1119(97)00060-7

M3 - Article

C2 - 9249060

AN - SCOPUS:0030744154

SN - 0378-1119

VL - 193

SP - 1

EP - 4

JO - Gene

JF - Gene

IS - 1

ER -

Cloning and analysis of the cDNA encoding the rod G-protein transducin α, β1 and γ1 subunits from the canine retina

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this