Cloning, analysis, and chromosomal localization of notch-1, a mouse homolog of drosophila notch

Francisco Franco del Amo, Maureen Gendron-Maguire, Pamela J. Swiatek, Nancy A. Jenkins, Neal G. Copeland, Thomas Gridley

Research output: Contribution to journalArticlepeer-review

91 Scopus citations

Abstract

The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.

Original languageEnglish (US)
Pages (from-to)259-264
Number of pages6
JournalGenomics
Volume15
Issue number2
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Genetics

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