Cloning, analysis, and chromosomal localization of notch-1, a mouse homolog of drosophila notch

Francisco Franco del Amo; Maureen Gendron-Maguire; Pamela J. Swiatek; Nancy A. Jenkins; Neal G. Copeland; Thomas Gridley

doi:10.1006/geno.1993.1055

Cloning, analysis, and chromosomal localization of notch-1, a mouse homolog of drosophila notch

Francisco Franco del Amo, Maureen Gendron-Maguire, Pamela J. Swiatek, Nancy A. Jenkins, Neal G. Copeland, Thomas Gridley

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Abstract

The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.

Original language	English (US)
Pages (from-to)	259-264
Number of pages	6
Journal	Genomics
Volume	15
Issue number	2
DOIs	https://doi.org/10.1006/geno.1993.1055
State	Published - Feb 1993

ASJC Scopus subject areas

Genetics

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title = "Cloning, analysis, and chromosomal localization of notch-1, a mouse homolog of drosophila notch",

abstract = "The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.",

author = "{del Amo}, {Francisco Franco} and Maureen Gendron-Maguire and Swiatek, {Pamela J.} and Jenkins, {Nancy A.} and Copeland, {Neal G.} and Thomas Gridley",

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T1 - Cloning, analysis, and chromosomal localization of notch-1, a mouse homolog of drosophila notch

AU - del Amo, Francisco Franco

AU - Gendron-Maguire, Maureen

AU - Swiatek, Pamela J.

AU - Jenkins, Nancy A.

AU - Copeland, Neal G.

AU - Gridley, Thomas

PY - 1993/2

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N2 - The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.

AB - The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.

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Cloning, analysis, and chromosomal localization of notch-1, a mouse homolog of drosophila notch

Abstract

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