Clinical significance of overexpressed cyclin-dependent kinase subunits 1 and 2 in esophageal carcinoma

J. J. Wang, Z. X. Fang, H. M. Ye, P. You, M. J. Cai, H. B. Duan, F. Wang, Z. Y. Zhang

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The mammalian cyclin-dependent kinase subunit (Cks) family has two members, Cks1 and Cks2. Overexpression of Cks1 and Cks2 has been reported to be associated with high aggressiveness and poor prognosis in several malignancies, including prostate and hepatocellular carcinomas. However, whether Cks1 and Cks2 are overexpressed in esophageal carcinoma remains uncharacterized. To investigate whether overexpression of the Cks family is clinically relevant in esophageal carcinoma, and whether expression patterns of Cks1 and Cks2 can serve as biomarkers for esophageal carcinoma. Real-time quantitative reverse transcription polymerase chain reaction, immunohistochemistry, and Western blot analyses were applied to detect the expression of Cks1 and Cks2 at the mRNA and protein levels, respectively. The associations between Cks1 or Cks2 expressions and clinical features and p27kip1 expressions in esophageal carcinoma were analyzed. Comparing with the adjacent noncancerous tissues, esophageal carcinoma exhibited elevated expression of Cks1 in 58% cases at the mRNA level and 54% cases at the protein level, and elevated expression of Cks2 in 65% cases at the mRNA level and 61% cases at the protein level, respectively. The expressions of both Cks1 and Cks2 were negatively associated with the p27kip1 protein level in the tumor tissues. Furthermore, overexpression of Cks1 and Cks2 in esophageal carcinoma was closely associated with poor pathological features of esophageal carcinoma, including higher histologic grade of tumor, regional lymph nodes invasion, and neoplastic embolus. Overexpression of Cks1 and Cks2 is associated with the aggressive tumor behaviors of esophageal carcinoma. Further efforts are needed to determine whether overexpression of Cks1 and Cks2 can serve as novel biomarkers for esophageal carcinoma.

Original languageEnglish (US)
Pages (from-to)729-736
Number of pages8
JournalDiseases of the Esophagus
Volume26
Issue number7
DOIs
StatePublished - Sep 2013

Keywords

  • Cks1
  • Cks2
  • Esophageal carcinoma
  • P27

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Clinical significance of overexpressed cyclin-dependent kinase subunits 1 and 2 in esophageal carcinoma'. Together they form a unique fingerprint.

Cite this