Clinical laboratory evaluation of the automicrobic system enterobacteriaceae biochemical card

J. R. Davis, C. E. Stager, R. D. Wende, S. M. Hussain

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The AutoMicrobic System Eneterobacteriaceae biochemical Card (AMS-EBC; Vitek Systems, Inc.) was evaluated in two clinical microbiology laboratories. A total of 502 consecutive clinical isolates representing members of the family enterobacteriaceae were tested in parallel with the AMS-EBC, API 20E, and Enterotube II systems. Discrepancies between systems were resolved with the conventional methods of Edwards and Ewing (P.R. Edwards and W.H. Ewing [ed.], Identification of Enterobacteriaceae, 1972) and Ewing and Martin (W.H. Ewing and W.J. Martin, in Manual of Clinical Microbiology, 1974) AMS-EBC correctly identified 96.6% and incorrectly identified 3.4% of the isolates. When 12 or more isolates of a species were evaluated, Serratia marcescens, Proteus mirabilis, and Enterobacter cloacae posed the greatest challenge to the system, with 92.6, 95.2, and 95.3%, respectively, being correctly identified. To confirm the accuracy of identification when all systems agreed, 93 randomly selected isolates were identified by conventional methods. The percent agreement was 100%. The reproducibility of triplicate determinations on 93 randomly selected isolates with AMS-EBC was 99.6%. The AMS-EBC was found to be an easy, rapid, and accurate method for identification of Enterobacteriaceae.

Original languageEnglish (US)
Pages (from-to)370-375
Number of pages6
JournalJournal of Clinical Microbiology
Volume14
Issue number4
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Microbiology (medical)

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