Clinical labeling and imaging of transplanted CD133+/CD34+ stem cells in patients with ischemic heart disease

Ali Ghodsizad; Viktor Bordel; Brian Bruckner; Mathias Loebe; Gunter Fuerst; I. Mirsaidi; M. Sucker; Arjang Ruhparwar; Matthias Karck; Hans Michael Klein

doi:10.1532/HSF98.20111138

Clinical labeling and imaging of transplanted CD133+/CD34+ stem cells in patients with ischemic heart disease

Ali Ghodsizad, Viktor Bordel, Brian Bruckner, Mathias Loebe, Gunter Fuerst, I. Mirsaidi, M. Sucker, Arjang Ruhparwar, Matthias Karck, Hans Michael Klein

Academic Institute

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The application of somatic stem cells has been shown to support the recovery of the myocardium in end-stage heart failure. A novel method for the intraoperative isolation and labeling of bone marrow-derived stem cells was established. After induction of general anesthesia, up to 400 mL of bone marrow were harvested from the posterior iliac crest and processed in the operating room under good manufacturing practice conditions by means of the automated cell-selection device Clini-MACS (Miltenyi Biotec). We subsequently injected autologous CD133+ and CD34+ stem cells in a predefined pattern around the laser channels in patients undergoing coronary artery bypass surgery and transmyocardial laser procedures. Intraoperative isolation and labeling is an effective cell-separation tool for the future, considering that novel cell markers can be promising new candidates for cell therapy.

Original language	English (US)
Pages (from-to)	E116-E118
Journal	Heart Surgery Forum
Volume	15
Issue number	2
DOIs	https://doi.org/10.1532/HSF98.20111138
State	Published - Apr 2012

ASJC Scopus subject areas

Surgery
Cardiology and Cardiovascular Medicine

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abstract = "The application of somatic stem cells has been shown to support the recovery of the myocardium in end-stage heart failure. A novel method for the intraoperative isolation and labeling of bone marrow-derived stem cells was established. After induction of general anesthesia, up to 400 mL of bone marrow were harvested from the posterior iliac crest and processed in the operating room under good manufacturing practice conditions by means of the automated cell-selection device Clini-MACS (Miltenyi Biotec). We subsequently injected autologous CD133+ and CD34+ stem cells in a predefined pattern around the laser channels in patients undergoing coronary artery bypass surgery and transmyocardial laser procedures. Intraoperative isolation and labeling is an effective cell-separation tool for the future, considering that novel cell markers can be promising new candidates for cell therapy.",

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AU - Ghodsizad, Ali

AU - Bordel, Viktor

AU - Bruckner, Brian

AU - Loebe, Mathias

AU - Fuerst, Gunter

AU - Mirsaidi, I.

AU - Sucker, M.

AU - Ruhparwar, Arjang

AU - Karck, Matthias

AU - Klein, Hans Michael

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AB - The application of somatic stem cells has been shown to support the recovery of the myocardium in end-stage heart failure. A novel method for the intraoperative isolation and labeling of bone marrow-derived stem cells was established. After induction of general anesthesia, up to 400 mL of bone marrow were harvested from the posterior iliac crest and processed in the operating room under good manufacturing practice conditions by means of the automated cell-selection device Clini-MACS (Miltenyi Biotec). We subsequently injected autologous CD133+ and CD34+ stem cells in a predefined pattern around the laser channels in patients undergoing coronary artery bypass surgery and transmyocardial laser procedures. Intraoperative isolation and labeling is an effective cell-separation tool for the future, considering that novel cell markers can be promising new candidates for cell therapy.

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Clinical labeling and imaging of transplanted CD133+/CD34+ stem cells in patients with ischemic heart disease

Abstract

ASJC Scopus subject areas

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