@article{e5863f89d6a24e988a27b90205cc5cbb,
title = "Classification of T-cell activation via autofluorescence lifetime imaging",
abstract = "The function of a T cell depends on its subtype and activation state. Here, we show that imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be used to classify the cells. T cells isolated from human peripheral blood and activated in culture using tetrameric antibodies against the surface ligands CD2, CD3 and CD28 showed specific activation-state-dependent patterns of autofluorescence lifetime. Logistic regression models and random forest models classified T cells according to activation state with 97–99% accuracy, and according to activation state (quiescent or activated) and subtype (CD3+CD8+ or CD3+CD4+) with 97% accuracy. Autofluorescence lifetime imaging can be used to non-destructively determine T-cell function.",
author = "Walsh, {Alex J.} and Mueller, {Katherine P.} and Kelsey Tweed and Isabel Jones and Walsh, {Christine M.} and Piscopo, {Nicole J.} and Niemi, {Natalie M.} and Pagliarini, {David J.} and Krishanu Saha and Skala, {Melissa C.}",
note = "Funding Information: We thank A. Movaghar for discussions of feature selection and machine learning classification methods and R. Schmitz for her assistance with formatting figures. This research was funded by the NIH (grant nos. R01 CA185747, R01 CA205101 and R01 CA211082, to M.C.S.); the Biotechnology Training Program of the National Institute of General Medical Sciences of the National Institutes of Health (no. T32GM008349, to K.P.M.); NIH awards (nos. R01DK098672 and R35GM131795, to D.J.P.; and T32DK007665, to N.M.N.); the NSF Graduate Research Fellowship Program (no. DGE-1747503, to K.P.M); and the National Science Foundation (no. EEC-1648035, to K.S.). Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2021",
month = jan,
doi = "10.1038/s41551-020-0592-z",
language = "English (US)",
volume = "5",
pages = "77--88",
journal = "Nature Biomedical Engineering",
issn = "2157-846X",
publisher = "Nature Publishing Group",
number = "1",
}