Circulating estradiol and hypothalamic corticotrophin releasing hormone enhances along with time after ovariectomy in rats: Effects of electroacupuncture

Hong Zhao, Zhanzhuang Tian, Yi Feng, Boying Chen

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Repeated electroacupuncture (EA) stimulation is known to enhance the circulating level of estrogen in the ovariectomized rats by stimulating the activity of the hypothalamus-pituitary-adrenal axis. To explore the mechanism of EA in promoting the homeostasis in ovariectomized rats, the hypothalamic CRH content and its mRNA expression as well as the circulating estradiol in the rats along with time after ovariectomy were detected. Female rats were divided into fourteen groups: 1 month after ovariectomy (OVX1M), OVX2M, OVX3M, OVX4M, OVX5M, OVX6M; intact 1 month (INT1M), INT2M, INT3M, INT4M, INT5M, INT6M; OVX1M with EA (OVX1M+EA) and intact 1 month with EA (INT1M+EA). The blood concentrations of estradiol (E2) and total contents of hypothalamic CRH were measured by radioimmunoassay. The expressions of CRHmRNA were semiquantified by RT-PCR. The results showed that the blood E2 concentrations increased gradually along with time after ovariectomy in the rats. The level was higher in OVX1M+EA than those in OVX1M, 2M, 3M (p < 0.05), which was near to the level in OVX6M. The total content (ng/g tissue) of hypothalamic CRH and the CRHmRNA expressions in the OVX6M rats were higher than other groups (p < 0.05). The CRH content or mRNA expression in the OVX1M+EA increased significantly compared with that in OVX1M (p < 0.05), and it was close to the level in the OVX4M and OVX5M. The results suggested that the circulating E2 and hypothalamic CRH enhances along with time after ovariectomy in rats, and the EA stimulation might speedup the process.

Original languageEnglish (US)
Pages (from-to)433-438
Number of pages6
JournalNeuropeptides
Volume39
Issue number4
DOIs
StatePublished - Aug 1 2005

Keywords

  • CRH
  • Estrogen
  • Ovariectomized rats

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Neurology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Biochemistry

Fingerprint Dive into the research topics of 'Circulating estradiol and hypothalamic corticotrophin releasing hormone enhances along with time after ovariectomy in rats: Effects of electroacupuncture'. Together they form a unique fingerprint.

Cite this